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  • Cytochemistry  (1)
  • Photomorphogenesis  (1)
  • brassinosteroids  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Cytochemical and histochemical characterization of cotyledonary bodies fromPharbitis nil seedlings (1996)
    Springer
    Protoplasma 191 (1996), S. 205-214 
    Details
    ISSN: 1615-6102
    Keywords: Cytochemistry ; Confocal scanning laser microscope ; Flowering ; Calcium indicator ; Gas chromatography/mass spectrometry ; Photomorphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytological and histochemical characterization of the structures from which an obscure substance is secreted via open stomata to the abaxial surface of Japanese morning glory (Pharbitis nil Choisy cv. Violet) cotyledons has been carried out. Observation of intact cotyledons using the light microscope revealed randomly distributed semi-transparent structures. These structures, which were shown to be the same as those previously described as giant oil cells are referred to here as cotyledonary bodies. These bodies can be easily isolated and purified after enzymatic digestion of the cotyledons. Using different staining procedures we have confirmed that each cotyledonary body originates from an individual mesophyJl cell during embryo development. Purified bodies consist of (i) a thick shell-like envelope; (ii) a transparent, hydrophilic zone; (iii) a hydrophobic core. Hydrophobic contents of the bodies were readily extracted with methanol and shown to contain fatty acids and phenolic compounds using the gas chromatography/mass spectrometry (GC/MS) technique. Methanolic extracts of cotyledonary bodies showed high fluorescence with two excitation and emission maxima. Using a fluorescence microscope we have shown that the bodies isolated from seedlings grown in continuous light, conditions non-inductive for flowering, and those grown under conditions inductive for flowering (a single 16 h, long dark period) have different fluorescence emission spectra. Different levels of free Ca2+ inside cotyledonary bodies isolated from light-grown and single dark-period treatedP. nil seedlings were found using the fluorescent calcium indicator dye Fluo-3 under a confocal scanning laser microscope. On the basis of these observations we speculate that cotyledonary bodies could be involved in floral induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281818
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The current status of physiology and biochemistry of brassinosteroids (1993)
    Springer
    Plant growth regulation 13 (1993), S. 147-159 
    Details
    ISSN: 1573-5087
    Keywords: brassinosteroids ; plant hormones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Brassinolide, first isolated from pollen of rape as a plant growth promoting substance, has been found to be widely distributed in the plant kingdom. Over thirty endogenous analogues, called collectively brassinosteroids, have been isolated and identified. As a new class of plant hormones, brassinosteroids show not only growth promoting activity but also other physiological effects on the growth and development of plants and draw attention as promising chemicals for practical application in agriculture. This review describes the current status of the studies on the natural occurrence, analysis, physiological actions, metabolism and biosynthesis of brassinosteroids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024257
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    Paper (German National Licenses)
    Fulltext
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