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  • 1
    ISSN: 1432-2048
    Keywords: Key words:Agrobacterium (6b gene) ; 6b gene ; Cytokinin resistance ; Nicotiana (transformed) ; Transgenic plant (tobacco)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The 6b gene of Agrobacterium tumefaciens has been demonstrated to modify the activity of the plant growth regulators, auxin and cytokinin. To study the possible mode of action of the gene, tobacco (Nicotiana tabacum L. cv. Samsun) plants were transformed with the A. tumefaciens C58-6b gene. Seeds obtained from morphologically normal transgenic as well as wild-type plants were germinated on media supplemented with growth-inhibitory levels of cytokinin, N6-benzyladenine (BA). The transgenic seedlings showed increased resistance to cytokinins, as reflected by continuous shoot development, whereas further growth of the wild-type plants beyond the cotyledonary stage was inhibited. Concurrently, the levels of 6b gene transcripts in transgenic seedlings increased greatly upon BA treatment. Since glucosylation of BA represents the main inactivation mechanism of the hormone, we analyzed BA glucoside formation during the early stages of seedling growth. Intracellular levels of the major BA metabolite, N6-benzyladenine-7-glucoside (80–92%), as well as other BA-derived components were found to be comparable in transgenic and wild-type seedlings. Therefore, increased resistance of the C58-6b transgenic seedlings to cytokinins could not be directly attributed to enhanced BA glucosylation and subsequent hormone inactivation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of superconductivity 11 (1998), S. 47-48 
    ISSN: 1572-9605
    Keywords: Flux dynamics ; vortex phase diagram ; irreversibility line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract Investigations on flux dynamics of ring-shaped T12Ba2CaCu2O8 superconducting thin films have been carried out by measuring the temperature dependent magnetization M(T) during field-cooling (FC) and zero-field-cooling (ZFC) processes. For a given magnetic field, from the magnetization behavior two distinct temperatures, Tkink and Tirr, can be defined. Below Tkink, a clear hysteretic behavior of M(T) is observed leading to a large irreversible signal ΔM = MFC − MZFC. Above Tkink, this irreversible signal, though being very small, is still non-zero until it eventually vanishes at a higher temperature Tirr. Above Tirr, both curves MFC(T) and MZFC(T) merge together and become temperature independent. We attribute the first region ( T〈 Tkink) to a 3D vortex-glass phase, the second region (Tkink 〈 T 〈 Tirr) to a vortex line liquid state and the third region (Tirr 〈 T 〈 Tc) to a pancake liquid state.
    Type of Medium: Electronic Resource
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