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  • 1
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of RAPD fragments amplified from mitochondrial DNA of male-sterile and male-fertile cytoplasm of sugar beet (Beta vulgaris L.) (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 273-278 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsBeta vulgaris ; Cytoplasmic male sterility ; RAPD ; Mitochondrial DNA ; Chloroplast DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondrial DNA fragments of two nearly isogenic lines of sugar beet (Beta vulgaris L.) were amplified by RAPD analysis. A number of fragments, most of them unique to either the male-sterile or the male-fertile cytoplasm, were selected for cloning and sequencing. One fragment was present in the PCR fingerprint pattern of both cytoplasms, whereas five of the selected fragments were specifically amplified from only one type of cytoplasm. The mitochondrial origin of all cloned RAPD fragments was confirmed by Southern hybridization. One fragment resulted in a hybridization pattern that suggests its repetitive presence in the mitochondrial genome of sugar beet. Four out of the five cytoplasm-specific RAPD fragments were shown to hybridize specifically to one type of cytoplasm only. One fragment hybridizing with the mtDNA from N-cytoplasm also revealed hybridization signals with both total and nuclear DNAs of N- as well as S-cytoplasm. Sequence alignments of this clone showed strong homologies with a part of the plastidal ndhC gene of higher plants, indicating that the male-fertile-specific mtDNA RAPD fragment is derived from chloroplast DNA. Sequence analysis of an amplified sterile-specific fragment revealed the presence of an open reading frame of 288 bp. Northern hybridization showed a transcription signal specific for the male-sterile cytoplasm. No sequence homology of the open reading frame to any known sequences was found. The results reveal an extremely high degree of sequence variability between the mtDNA of the N- and S-cytoplasm of Beta vulgaris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050410
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    DNA fragments of organellar origin in random amplified polymorphic DNA (RAPD) patterns of sugar beet (Beta vulgaris L.) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 775-779 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; RAPD ; Mitochondrial DNA ; Chloroplast DNA ; Cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The technique of random amplified polymorphic DNA (RAPD) offers a broad range of applications in the investigation of plant genomes. A promising prospect is the use of RAPD products as genetic markers. We have investigated a possible organellar source of fragments in RAPD patterns of total DNA. Two nearly-isogenic lines of cytoplasmic male-sterile and male-fertile sugar beet (Beta vulgaris L.) were subjected to RAPD analysis with six different primers. Total, nuclear, mitochondrial (mt), and chloroplast (cp), DNA from each line were investigated. Reproducible DNA fingerprints could be obtained from both organellar DNAs. Differences in band patterns of mtDNA between cytoplasmic male-sterile and -fertile lines were observed with five out of six primers, whereas different cpDNA patterns were generated by one of the primers. Consequently, the RAPD technique can be used to discriminate between different cytoplasms. Clear evidence is provided for the organellar origin of fragments in genomic (total DNA) RAPD patterns. The consequences of these results for the interpretation of RAPD analyses are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253985
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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