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  • cat  (3)
  • DAPI/actinomycin D  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 45-53 
    ISSN: 1432-2242
    Keywords: Heterochromatin ; DAPI/actinomycin D ; Salmo gairdneri R. ; Salmo trutta fario L. ; Salvelinus fontinalis M.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The heterochromatins of rainbow trout (Salmo gairdneri R.), brown trout (Salmo trutta fario L.) and brook trout (Salvelinus fontinalis M.) were characterized by sequential chromomycin A3/distamycin A/DAPI (CDD) and DAPI/actinomycin D (DAPI/AmD) fluorescence. On most biarmed chromosomes, an equilocal localization of prominent DAPI/AmD positive, chromomycin A3 negative, AT-rich blocks at the centromeres were observed in all three species. Band karyotypes of the three species were established. In rainbow trout, several DAPI/AmD positive heterochromatin blocks behaved positive in a silver-staining method. Mitotic and interphase studies proved the presence of inter-individual NOR variation in brown trout. The NORs of brook trout were localized on chromosomes 5, 10, 14, 15 and 29.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Veterinary research communications 24 (2000), S. 115-124 
    ISSN: 1573-7446
    Keywords: cat ; genes ; mutation ; PCR ; p21 WAF1 ; p27 Kip1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The coding regions of tumour suppressor and cell cycle regulatory genes p21 WAF1 and p27 Kip1 were investigated in 101 feline tumours of various types. No damaging mutations were present in the analysed areas of the genes
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Veterinary research communications 22 (1998), S. 249-255 
    ISSN: 1573-7446
    Keywords: cat ; mutation ; p53 gene ; sarcoma ; tumour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Twenty feline neoplasms were sequenced in the region from exons 5 to 8 for the presence of tumour suppressor gene p53 mutations. In a spindle cell sarcoma of the bladder, a missense mutation (codon 164 AAG→GAG, lysine→glutamic acid) in exon 5 was detected. In a pleomorphic sarcoma, a 23 bp deletion involving the splicing junction between intron 5 and exon 6 was observed. In a fibrosarcoma, a 6 bp deletion of p53 covering 2 bp of exon 7 and 4 bp of intron 7, including the splicing junction, was found. The study demonstrates three new p53 mutations in different types of sarcomas in cats.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Veterinary research communications 22 (1998), S. 21-29 
    ISSN: 1573-7446
    Keywords: cat ; cytochrome ; cytogenetics ; mesenchymal ; neoplasm ; tumour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Four mesenchymal neoplasms (two malignant fibroblastic histiocytomas, one fibrosarcoma, one fibroma) were investigated cytogenetically. Although no specific alterations were present, the frequent nonrandom involvement of chromosome E1 became clearly evident. Karyotypic alterations involving unrelated clonal changes were also present in the fibroma.
    Type of Medium: Electronic Resource
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