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  • 1
    Electronic Resource
    Electronic Resource
    The occurrence of dark neurons in the normal and deafferentated lateral vestibular nucleus in the rat (1975)
    Springer
    Acta neuropathologica 31 (1975), S. 117-127 
    Details
    ISSN: 1432-0533
    Keywords: Dark Neurons ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The lateral vestibular nucleus was studied by light and electron microscopy in normal rats as well as in rats in which the anterior cerebellar vermis was destroyed. Dark neurons were seen in many of the operated rats but were rarely found in normal control animals. The dark neurons were not seen in adjacent nuclei. In additional rats, it was found that anoxia, extra anaesthetic, postmortem rough handling, and sham operations did not increase the frequency of dark neurons. These data indicate that dark neurons might not always be artifactual and that the lateral vestibular nucleus appears to be a focal point for their occurrence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688146
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Transgenesis in mice by cytoplasmic injection of polylysine/DNA mixtures (1995)
    Springer
    Transgenic research 4 (1995), S. 353-360 
    Details
    ISSN: 1573-9368
    Keywords: microinjection ; transgenesis ; polylysine ; DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pronuclear injection is currently the most often used method to make transgenic animals, but in some animal species it is temporally restrictive due to difficulty in visualizing pronuclei. However, the injection of construct DNA into the cytoplasm does not result in transgenesis. The production of transgenic mice by a cytoplasmic microinjection technique of polylysine complexed DNA into pronuclear stage zygotes is described. Transgenic mice were produced from cytoplasmic microinjection of mixtures of a 5.3 kb linearized DNA and poly-l-lysine (degree of polymerization=51). Effects on transgenic frequency of both the lysine to phosphate ratio of polylysine to DNA and DNA concentration were studied. About 12.8% of the pups born from zygotes cytoplasmically microinjected with a polylysine/DNA mixture having a lysine to phosphate ratio (L:P) of 1∶1 microinjection positive control of DNA alone was 21.7%. No transgenic pups were born from microinjection of DNA alone into the cytoplasm. Complexes of polylysine/DNA were detected using agarose gel electrophoresis at the conditions which produced transgenic mice. The presence of polylysine with construct DNA altered thein vitro activities of restriction endonuclease and DNA ligase on the construct DNA. The production of transgenic animals using DNA and polylysine in the absence of any other signal protein suggests that a DNA/polylysine complex but not DNA alone can act as a substrate for transgenesis from the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01973753
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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