ISSN:
1432-0584
Schlagwort(e):
DNA
;
analysis
;
Gene rearrangement
;
PCR
;
Fixed material
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary In the present study methods are described for the analysis of the genomic structure of DNA isolated from tissues that have been stored up to several years as air-dried or stained bone marrow smears, bone marrow biopsies in “Histicon”, chromosomal preparations in fixative or as formalin fixed tissues embedded in paraffin. By application of the new techniques clonal B- and T-cell disorders, Philadelphia chromosome translocations and ras oncogene mutations in pancreatic carcinomas could be detected. Thus, these DNA extraction procedures may open new avenues to pathological archives and enable the analysis of samples when fresh material is not available.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01702923
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