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  • 1
    Electronic Resource
    Electronic Resource
    Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs) (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 985-990 
    Details
    ISSN: 1432-2242
    Keywords: Grapevine ; Microsatellite DNA ; DNA polymorphisms ; STS ; SSLP ; STMS ; Vitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detecting high heterozygosity (69%–88%) within individual grapevine cultivars and high genetic variation between cultivars, making it a useful marker type for plant genome mapping and genome typing. For five loci a screening of 26 V. vinifera cultivars found 13, 12, 8, 5, and 4 different length alleles respectively with some alleles more common than others. The genomic DNA sequences surrounding microsatellite sequences were conserved within the genus permitting STS primers to amplify STSs from other Vitis species. These Vitis species were found to have some unique alleles not present in V. vinifera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00211051
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 173-180 
    Details
    ISSN: 1432-2242
    Keywords: DNA fingerprinting ; Grapevine ; RFLP ; rDNA ; Satellite DNA ; Vitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Repetitive DNA sequences present in the grapevine genome were investigated as probes for distinguishing species and cultivars. Microsatellite sequences, minisatellite sequences, tandemly arrayed genes and highly repetitive grapevine sequences were studied. The relative abundance of microsatellite and minisatellite DNA in the genome varied with the repeat sequence and determined their usefulness in detecting RFLPs. Cloned Vitis ribosomal repeat units were characterised and showed length heterogeneity (9.14–12.15 kb) between and within species. A highly repetitive DNA sequence isolated from V. vinifera was found to be specific only to those species classified as Euvitis. DNA polymorphisms were found between Vitis species and between cultivars of V. vinifera with all classes of repeat DNA sequences studied. DNA sequences suitable for DNA fingerprinting gave genotype-specific patterns for all of the cultivars and species examined. The DNA polymorphisms detected indicates a moderate to high level of heterozygosity in grapevine cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222076
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A hypervariable middle repetitive DNA sequence from citrus (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1248-1252 
    Details
    ISSN: 1432-2242
    Keywords: DNA fingerprinting ; Citrus ; Hypervariable DNA ; Cultivar typing ; Somatic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The use of hypervariable sequences for DNA typing of plants is focussed on microsatellites and on amplification of regions defined by random (RAPD) or defined (AFLP) primers for PCR analysis of genomes. A hypervariable length of middle repetitive DNA has been isolated from citrus that contains no obvious hypervariable structures. The fingerprinting probe was shown to have an important commercial application in the separation of zygotic from nucellar progeny. A somatic variant of the sequence within one orange tree suggests that somatic variation in hypervariable markers may be a common event.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220936
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    Paper (German National Licenses)
    Fulltext
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