ISSN:
1617-4623
Keywords:
Bent DNA
;
DNA structure
;
Saccharomyces cerevisiae
;
2 μm circle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Until recently it was assumed that any short segment of DNA could be approximated as a straight rod. Many instances, however, have been reported in which the helical axis is curved. We have devised a simple method for selective identification of DNA segments containing a sequence-directed bend (curvature), by means of a two-dimensional polyacrylamide gel electrophoresis. In order to gain general insights into the structural features and the functional significance of sequence-directed bends, a bank of plasmids carrying bent DNA inserts from the Saccharomyces cerevisiae total genomic DNA was constructed. Primary characterizations of a set of bent DNA segments randomly cloned from S. cerevisiae are presented. One of the cloned DNA segments appears to be derived from a yeast plasmid, the 2 μm circle DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00337718
Permalink
