ISSN:
1432-0738
Keywords:
Key words HPLC
;
Mouse bioassay
;
DSP toxins
;
Okadaic acid
;
Time
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The progressive increase of DSP toxic episodes in shellfish in the last few years has generated a series of criticisms centered on the suitability of the mouse bioassay as a reference method to regulate the harvest of mussels from the growth area. The observation time in injected mice is currently fixed in 12 h by the actual Spanish rules. The revision of this time period and the lack of a established DSP toxin threshold which permits the commercialization of mussels contaminated under certain levels, are some of the actual demands from the industry. In this study, the results obtained in a comparative study of DSP toxic mussels are shown using the HPLC method and the mouse bioassay. Based on these results, we consider feasible the reduction to 5 h of the observation times of the mouse bioassay currently established in the actual legislation, as well as the establishment of a DSP-toxin threshold of 2 μg okadaic acid/g hepatopancreas, which regulates the possibility of harvesting and commercialization of contaminated mussels.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002040050296
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