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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 260 (1998), S. 475-479 
    ISSN: 1617-4623
    Keywords: Key wordsMycobacterium smegmatis ; Dormancy ; Histone-like protein ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aerobic saprophyte Mycobacterium smegmatis, like its pathogenic counterpart M. tuberculosis, has the ability to adapt to anaerobiosis by shifting down to a dormant state. Here, we report the identification and molecular genetic characterisation of the first dormancy-induced protein in M. smegmatis. Comparative SDS-polyacrylamide gel electrophoresis of protein extracts of aerobically growing and dormant anaerobic M. smegmatis cultures revealed the upregulation of a 27-kDa protein in the dormant state. Peptide sequencing showed that the induced protein is a homologue of the histone-like protein Hlp, predicted by the M. tuberculosis genome project. The corresponding hlp gene was cloned from M. smegmatis and sequenced. Disruption of the hlp gene eliminated the histone-like protein but did not affect the viability of the dormant culture.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 1 (1986), S. 209-217 
    ISSN: 1476-5535
    Keywords: Lactic acid bacteria ; Lactobacilli ; Streptococcus ; Homofermentative bacteria ; Heterofermentative bacteria ; Leuconostoc ; Dairy microbiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A rapid and simple technique utilizing the APIZYM enzymatic patterns complemented with arginine dihydrolase and citratase was developed for species differentiation of 40 lactic acid bacteria relevant to the dairy industry.Streptococcus species in general produced no β-galactosidase, except forStreptococcus thermophilus. Lactobacillus species showed strong aminopeptidases and galactosidases but contained no arginine dihydrolase and citratase. Among the group N-streptococci,Streptococcus diacetylactis produced citratase, whereasStreptococcus cremoris differed by the production of butyrate esterase.Streptococcus faecalis was readily distinguishable fromStreptococcus lactis by butyrate esterase activity that was the basis of the differential agar developed. Heterofermentative lactobacilli differed from homofermentative lactobacilli in possessing arginine dihydrolase and citratase but by not producing leucine-aminopeptidase.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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