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Cloning and sequencing of a Deleya marina gene encoding for alginate lyase (1999)

Kraiwattanapong, J. ; Tsuruga, H. ; Ooi, T. ; [et al.]

Springer

Biotechnology letters 21 (1999), S. 169-174

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ISSN: 1573-6776

Keywords: alginate lyase ; alginic acid ; Deleya marina

Source: Springer Online Journal Archives 1860-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract A bacterial strain N-1 was isolated as a decomposer of alginate and identified as Deleya marina. The alyA encoding for alginate lyase was cloned into Escherichia coli. The structural gene, located on a 1.9-kb SalI fragment, revealed 1,122 bp encoding a mature protein of 348 amino acids and a signal peptide of 26 amino acids. The deduced amino acid sequence of the D. marina alginate lyase showed high homology to AlgL of Pseudomonas aeruginosa with 63% identity and belonging to class 1 by hydrophobic cluster analysis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1005435725903

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