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  • Depression  (1)
  • Diagnostic ELISA system  (1)
  • 1
    ISSN: 1432-069X
    Keywords: Key words Bullous pemphigoid antigen 1 and 2 ; Antigenic epitopes ; Recombinant bacterial proteins ; Diagnostic ELISA system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Specific antibodies directed against special hemidesmosomal proteins are involved in the pathogenesis of bullous pemphigoid (BP), and detection of these antibodies is crucial for a correct diagnosis. As the BP autoantigen primary structures are known, the question was addressed as to whether it is possible to demonstrate circulating antibodies against BP autoantigens (BPAG1 and BPAG2) by means of an ELISA system, using antigenic epitopes. With the help of the programs Peptidestructure and Plotstructure, antigenic epitopes of BP antigens were predicted, chemically synthesized and screened using serum from ¶43 proven BP patients. The coding sequences of the best antigenic epitopes were then chemically synthesized and inserted as monomer and homo- or hetero-oligomer forms into fusion-expression plasmids (PGEX-4T, Pharmacia) in-frame to the C-terminus of glutathione-S-transferase. Fusion products were expressed and purified from Escherichia coli cells by affinity chromatography. The recombinant proteins were used for the detection of antibodies in the serum of 43 BP patients and of 60 controls (including 30 healthy persons, 22 patients with pemphigus vulgaris and 8 patients with other bullous dermatoses). Use of the homo- and hetero-oligomers of the recombinant fusion peptides increased the sensitivity of the disease-specific antibody detection. When a mixture of the best recombinant fusion proteins was used, the sensitivity of the ELISA assays in the case of the BP patients’ serum was 0.90. This system could form the basis of a rapid and simple system for the diagnosis of BP.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European archives of psychiatry and clinical neuroscience 218 (1974), S. 107-113 
    ISSN: 1433-8491
    Keywords: Atropine Coma Therapy ; Intelligence ; Memory ; Anxiety ; Depression ; EEG ; Atropin-Koma-Behandlung ; Intelligenz ; Gedächtnis ; Depression und Angst ; EEG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Verff. führten testpsychologische Untersuchungen an 20 psychiatrischen Kranken im Laufe von Atropin-Koma-Behandlungen durch. Aufgrund der Beobachtungsergebnisse und anhand von Literaturangaben werden folgende Schlußfolgerungen gezogen: 1. Durch die Atropin-Koma-Behandlung werden die intellektuellen oder Gedächtnisleistungen nicht vermindert. Auch die hirnelektrische Tätigkeit wird nicht beeinflußt. Die psychischen Leistungen verbessern sich sogar nach durchgeführter Therapie. 2. Die Atropin-Koma-Behandlung entfaltet in Krankheitsbildern, die durch Depression und Angst charakterisiert werden, eine bessere therapeutische Wirksamkeit als die Elektroschock-Behandlumg. 3. Bei paranoiden Krankheitsbildern dagegen scheint sie therapeutisch wirkungslos zu sein.
    Notes: Summary Psychological tests were administered to 20 mental patients during and after atropine coma therapy. The results of the study and data in the literature indicate that: 1. Atropine coma therapy does not modify the electrical activity of the brain or the level of intelligence or memory. In fact, mental ability is better after the treatment than before. 2. Atropine coma therapy is more effective in the anxiety-depression syndrome than electroconvulsive treatment. 3. In the paranoid syndrome, in contrast, it does not seem to have any effect.
    Type of Medium: Electronic Resource
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