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  • 1
    ISSN: 1573-1561
    Keywords: Foveal glands ; sex pheromone gland ; 2,6-dichlorophenol ; Dermacenter andersoni ; Dermacentor variabilis ; tick ; Ixodidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The foveal glands of the Rocky Mountain wood tick,Dermacentor andersoni Stiles, are the sex pheromone glands from which the sex pheromone is released via the foveae dorsales. The sex pheromone, 2,6-dichlorophenol, was recovered from extracts of these glands by GLC. Other evidence of the role of these glands in sex pheromone production is described. A36Cl-labelled volatile compound (or compounds) was (were) collected from partially engorged femaleD. andersoni fed in36Cl-labelled hosts, but no labelled compounds were collected when the foveae dorsales were blocked. X-ray analysis revealed unusual concentrations of chlorine in the foveal glands compared to other tissues. Autoradiography also revealed significant accumulations of radiochlorine in the vicinity of these glands. Presumably, the foveal glands of the American dog tick,Dermacentor variabilis (Say), are the sex pheromone glands of that species also, since a36Cl-labelled volatile was collected from female ticks fed on a36Cl-labelled host. However, attempts to recover 2,6-dichlorophenol from gland extracts or volatile emissions fromD. variabilis were unsuccessful.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Hylurgopinus rufipes ; Scolytus multistriatus ; Coleoptera ; Scolytidae ; sesquiterpenes ; host attractants ; Dutch elm disease ; kairomone ; pheromone ; elm bark beetles ; Ulmus americana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Hylurgopinus rufipes male and female beetles were attracted to American elms infected with Dutch elm disease, and to American elms killed by injection of cacodylic acid.H. rufipes was also attracted to solvent extracts of elm, or to Porapak Q-trapped volatiles from elm. The major components of attractive fractions of Porapak Q-trapped volatiles were isolated, identified, and tested in field bioassays. Several artificially compounded mixtures of sesquiterpenes were attractive toH. rufipes, although no bait tested was as attractive as diseased tree controls. Laboratory bioassays withH. rufipes were marginally successful. In laboratory bioassays, nine of 14 sequiterpenes identified from active fractions of Porapak extracts elicited significant response fromScolytus multistriatus male and female beetles: δ- and γ-cadinene, α-cubebene, γ-muurolene, and β-elemene were most active. However, in field tests, none of the sesquiterpenes alone or in combination significantly attractedS. multistriatus, nor did they significantly enhance the attraction ofS. multistriatus to female-produced pheromone components (4-methyl-3-heptanol [H] and α-multistriatin [M]). In other field tests, α-cubebene (C) significantly enhanced response ofS. multistriatus to H plus M, but foliage, logs, or chips of healthy elm did not enhance trap catch to HMC.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-1561
    Keywords: Dermacentor variabilis ; Acari ; Ixodidae ; tick ; American dog tick ; sex pheromone ; 2,6-dichlorophenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract No detectable sex pheromone, 2,6-dichlorophenol (2,6-DCP), was found inDermacentor variabilis engorged nymphs, teneral females, replete females, or unfed males. The amount of 2,6-DCP present in unfed females of different ages was extremely variable; no relationship between age and 2,6-DCP content in these unfed females was observed. Partially fed virgin females had less 2,6-DCP than unfed females, but there was no change in pheromone content during the course of feeding. Sex pheromone was also found in partially fed males.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-1561
    Keywords: Rocky Mountain wood tick ; dog tick ; Dermacentor andersoni ; Dermacentor variabilis ; sex pheromone ; 2,6-dichlorophenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract 2,6-Dichlorophenol is the only active sex attractant component detected in the extracts of the Rocky Mountain wood tick,Dermacentor andersoni Stiles and the American dog tick,Dermacentor variabilis (Say). It elicits from the male of each species a hierarchy of responses culminating in copulation. This compound probably occurs generally throughout the metastriate Ixodidae. 2,6-Dibromophenol, an artifact, also elicits the same sexual responses from the wood tick, but phenol andp-cresol do not.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-1561
    Keywords: American dog tick ; Dermacentor variabilis ; Acarina ; Ixodidae ; sex pheromone ; mating disruption ; 2,6-dichlorophenol ; control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Treatment ofD. variabilis-infested dogs with formulations of the sex pheromone, 2,6-dichlorophenol, significantly reduced mating by the attached ticks. Aqueous emulsions of a gelatin microcapsule-xylene slurry and a pheromone-loaded, molecular-sieve powder were used. Concentration was an important variable influencing product efficacy. Such formulations combined with an acaricide may prove effective in reducing tick populations on livestock and pets.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-1561
    Keywords: Trypodendron lineatum ; lineatin ; pheromone ; attractant ; tricyclic acetal ; Scolytidae ; aggregation pheromone ; ambrosia beetle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An attractant compound was isolated from frass produced byTrypodendron lineatum female beetles boring in Douglas fir. The proposed structure is one of two isomeric tricyclic acetals, to which the trivial name lineatin is assigned.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-1561
    Keywords: Pinus ponderosa ; Dendroctonus brevicomis ; Temnochila chlorodia ; pheromone ; kairomone ; exo-bievicomm ; frontalin ; trans-ver-benol ; verbenone ; terpenes ; Coleoptera ; Scolytidae ; Trogositidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The responses of the western pine beetle (Dendroctonus brev-icomis LeConte) andTemnochila chlorodia (Mannerheim) to candidate attractants—exo- andendo-brevicomm, frontalin,trans-verbenol, ver-benone, and ponderosa pine turpentine and its major monoterpene components—were quantified by counts of beetles on traps baited with the various attractants, singly and in combinations released simultaneously. Combinations ofexo-brevicomin and frontalin plus a monoterpene or turpentine were the most attractive toD. brevicomis. The responses to these attractant combinations were reduced when verbenone plustrans-verbenol were present. All single compounds and binary mixtures, exceptexo-brevicomin plus frontalin, were much less attractive.exo-Brevicomin was most attractive toT. chlorodia, and this response appeared to decrease when verbenone plustrans-verbenol were present.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-1561
    Keywords: Ips pini ; Coleoptera ; Scolytidae ; aggregation pheromone ; ipsdienol ; interpopulational variation ; hybrid ; genetic control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ips pini from Idaho (ID) produce (−)-ipsdienol and respond to it in the laboratory.I. pini from New York (NY) produce (+) and (−)-ipsdienol in a 65∶35 ratio and respond much more strongly to the (+) than to the (−) enantiomer. Response byI. pini-ID to (−)-ipsdienol in the laboratory was inhibited by (+)-ipsdienol, while the field response byI. pini-NY was optimal to a 50:50 mixture of (+) and (−)-ipsdienol. This differential production and response to enantiomers of ipsdienol is sufficient to account for populational specificity observed in earlier experiments and confirmed by our current work. Reciprocal hybrids of the NY and IDI. pini populations did not differ in their patterns of attraction and response; those of both types of F1 were similar to NY beetles. Thus, it appears that (+)-ipsdienol is produced by the hybrids and is necessary for their maximum response, but this supposition was not tested. The genes controlling the pheromone biology ofI. pini are not sex linked. Since previous whole-antenna and single-cell electrophysiological data show that the receptor systems of the two populations are virtually identical, response behavior seems to be governed by the central nervous system rather than by the characteristics of the olfactory receptors.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-1561
    Keywords: Dermacentor variabilis ; Dermacentor andersoni ; 2,6-dichlorophenol ; sex pheromone ; neutral lipid ; sex pheromone glands ; ticks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Evidence is presented indicating the presence of the tick sex pheromone, 2,6-dichlorophenol in lipid droplets in the foveal glands ofDermacentor variabilis (Say) andDermacentor andersoni Stiles. The pheromone appears to be dissolved in the lipid droplets. The droplets consist of neutral lipids, mostly triacylglycerides and cholesterol esters. The esterified fatty acid profiles of foveal gland triacylgylcerides are different from those of other tissues examined or reported for other ticks. The percentage of shorter chain, mostly saturated fatty acids were decreased, while the longer chain polyunsaturated fatty acids were increased. The biological advantages of lipid solution for storage, translocation, and release of the sex pheromone from the female tick are discussed.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-1561
    Keywords: Dermacentor variabilis ; Acari ; Ixodidae ; tick ; American dog ; tick ; pheromone ; genital sex pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The occurrence of a genital sex pheromone in the anterior reproductive tract of partially fed femaleDermacentor variabilis was demonstrated by extraction and bioassay. A new type of bioassay, the “neutered” female assay, was developed to test the potency of extracts or chemically defined fractions to stimulate males to copulate. Electrophysiological tests confirmed the ability of males to detect the pheromone with sensilla on their cheliceral digits. Males of bothD. variabilis andD. andersoni exhibited neuronal excitation when stimulated with extracts of theD. variabilis reproductive tissues. The pheromone, which is soluble in methanol, was fractionated and found to contain at least two fractions that stimulated copulation by sexually excited males. Evidently, the pheromone is a mixture of two or more compounds. Histologic, ultrastructural, and histochemical studies suggest the vestibular vagina as the site of genital sex pheromone occurrence, presumably from secretions of the surrounding lobular accessory gland. The identity of the compounds that comprise the pheromone remains unknown.
    Type of Medium: Electronic Resource
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