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  • 1
    Electronic Resource
    Electronic Resource
    The use of heteronuclear cross-polarization for backbone assignment of 2H-, 15N- and 13C-labeled proteins: A pulse scheme for triple-resonance 4D correlation of sequential amide protons and 15N (1995)
    Springer
    Journal of biomolecular NMR 5 (1995), S. 323-326 
    Details
    ISSN: 1573-5001
    Keywords: Heteronuclear NMR ; Deuterium labeling ; Cross-polarization ; Triple-resonance experiment ; Pulse scheme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A new four-dimensional pulse scheme is described for the main-chain assignment of proteins by means of the J connectivity of the amide proton and nitrogen resonances of adjacent residues. Since the new experiment, 4D CP-HN(COCA)NH, involves heteronuclear cross-polarization for magnetization transfer from 13C=O to 15N via 13Cα, a relatively strong WALTZ-16 decoupling rf field is applied to 13Cα during magnetization transfer. Consequently, 13Cα is effectively decoupled from its attached 2H in the case of deuterated proteins, in the absence of a decoupling rf field for 2H. This efficiently improves the sensitivity of the experiment through 13C line narrowing. The experiment was performed on a randomly 60% deuterated protein, and the sensitivity of the final 4D spectrum was found to be excellent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00211761
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  • 2
    Electronic Resource
    Electronic Resource
    Preliminary proton nuclear magnetic resonance studies of human saliva (1991)
    Springer
    Oral radiology 7 (1991), S. 7-11 
    Details
    ISSN: 1613-9674
    Keywords: Proton NMR ; Human parotid saliva ; Human submandibular saliva
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Summary-Proton NMR spectra of gustatory stimulated healthy human whole, parotid and submandibular and sublingual saliva were measured. The typical patterns of their spectra were obtained. Among these three kinds of fluids the parotid saliva which contains preferentially serous saliva presented a relatively well resolved spectrum with satisfactory signal to noise ratio in a given short time(30min.). For eight subjects of parotid saliva collected in the afternoon the marked increase in signal intensity in the methyl and methylene proton region of proteins(peptides)was observed as compared with those collected in the morning, reflecting the circadian rhythms in the protein concentration in saliva. On the other hand the methyl peak of lactic acid presented the opposite tendency, which might be also correlated with the circadian rhythms of the glucose metabolism of the parotid gland. The proton NMR spectra of three patients suspected of salivary gland disease were different from those of healthy subjects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02351673
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    Paper (German National Licenses)
    Fulltext
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