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  • 1
    Electronic Resource
    Electronic Resource
    The aromatic amino acid content of the bacterial chaperone protein groEL (cpn60) Evidence for the presence of a single tryptophan
    Amsterdam : Elsevier
    FEBS Letters 292 (1991), S. 9-12 
    Details
    ISSN: 0014-5793
    Keywords: Chaperone protein ; Fluorescence ; GdnHCl ; Spectrophotometry ; circular dichroism ; e d ; guanidinium chloride
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-5793(91)80821-J
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The aromatic amino acid content of the bacterial chaperone protein groEL (cpn60) Evidence for the presence of a single tryptophan
    Amsterdam : Elsevier
    FEBS Letters 292 (1991), S. 9-12 
    Details
    ISSN: 0014-5793
    Keywords: Chaperone protein ; Fluorescence ; Spectrophotometry
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-5793(91)80821-J
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Diabetic glomerulopathy in the uninephrectomized rat resists amelioration following islet transplantation (1982)
    Springer
    Diabetologia 23 (1982), S. 347-353 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes ; uninephrectomy ; islet transplantation ; glomerulus ; mesangium ; basement membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Uninephrectomy is known to accelerate the development of both functional and morphological changes seen with experimental diabetic nephropathy in the rat. The present experiments utilized electron and light microscopic morphometric techniques to assess glomerular basement membrane width and the volumes of the total mesangium and its cellular and matrix components of inbred Lewis rats made diabetic at 6 weeks of age and uninephrectomized 9 days later. Immunofluorescent microscopy was used to evaluate IgG and C3 in the mesangium. The reversibility of established diabetic glomerular lesions in uninephrectomized diabetic rats after 7 months of diabetes was studied by performing intraportal transplant of neonatal pancreatic tissue. Renal biopsies were taken 2 months later in transplanted and non-transplanted animals. Islet transplantation lowered plasma glucose to normal levels (29.6 to 7.3 mmol/l) and raised plasma insulin values (6.3 to 53 μU/l). Glomerular basement membrane width in transplanted rats (268 nm) still exceeded the same measure (226 nm) in nondiabetic uninephrectomized rats. In transplanted animals volumes of the mesangium (0.51 × 106 μm3) and of its cellular (0.27 × 106 μm3) and matrix (0.24 × 106 μm3) components remained higher than similar measures in control rats (0.32 × 106, 0.17 × 106 and 0.15 × 106 μm3, respectively). Mesangial IgG in treated animals approached normal, but mesangial C3 remained similar to levels in non-transplanted diabetic control animals. These observations in uninephrectomized-diabetic rats contrast with previous observations in intact diabetic rats in which mesangial volumes and localization of immunoglobulins and complement returned to normal levels following islet transplantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253743
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Failure to find amyloidosis in dogs treated with long-term intravenous insulin delivered by a totally implantable pump (1983)
    Springer
    Diabetologia 25 (1983), S. 448-450 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes ; insulin ; amyloidosis ; dogs ; kidney
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined tissues of seven non-diabetic mongrel dogs and four diabetic beagle dogs treated with constant insulin infusion via totally implantable pumps for from 210 to 880 days. Kidney and skeletal muscle tissue from all dogs were stained with Congo Red and thioflavin-T and appropriately examined. Kidney tissues from the beagle dogs were examined by electron microscopy. No amyloid deposits were found in any of these tissues. Thus, we cannot confirm an earlier report of amyloid occurring in dogs given long-term intravenous insulin. It is concluded that amyloidosis is not a necessary complication of long-term intravenous insulin infusion in dogs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282527
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Indices of renal structure and function in patients with type 1 diabetes mellitus (1992)
    Springer
    Acta diabetologica 29 (1992), S. 119-122 
    Details
    ISSN: 1432-5233
    Keywords: Diabetes ; Glomerulus ; Mesangium ; Nephropathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The evaluation of renal biopsies from diabetic patients rests upon measurements of many structures in glomeruli, the tubules and the interstitium. These individual values can also be expressed as an index of renal structure. To assess the validity of the individual values versus a published index of renal structure, we evaluated glomerular basement membrane width, mesangial volume fraction, other measures of glomerular structure, and percentage glomerulosclerosis in type 1 diabetic subjects with renal function ranging from normal to marked albuminuria, hypertension, and a falling glomerular filtration rate. For all measured structural parameters and for the index, all groups of diabetic patients, even those who had completely normal renal function, had values different from non-diabetic subjects (P〈0.001). Furthermore, there was an excellent correlation between the structural index and a similar functional index (r=0.77).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00573475
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    Paper (German National Licenses)
    Fulltext
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