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  • Dichloromethylene diphosphate  (1)
  • Hydronephrosis  (1)
  • Near-infrared spectroscopy  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Nitrosyl hemoglobin detected by near-infrared spectroscopy in rat liver allografts (1999)
    Springer
    Transplant international 12 (1999), S. 307-315 
    Details
    ISSN: 1432-2277
    Keywords: Key words Nitric oxide ; Near-infrared spectroscopy ; Nitrosyl hemoglobin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Near-infrared spectroscopy (NIRS) is a noninvasive biomeasurement system with rays in the near-infrared region that possess high permeability to biological tissues. NIRS was applied to liver allografts undergoing rejection in rats treated with deoxyspergualin (DSG) or tacrolimus (FK506). The nitrosyl hemoglobin (Hb) levels detected in the liver grafts increased 3 days and 5 days after grafting in both allogeneic and syngeneic transplantation. The levels on day 8 remained high in the allogeneic graft, but markedly decreased in the syngeneic graft. Although the serum levels of nitrite and nitrate were extremely low 8 days after grafting in allografted recipients treated with DSG or FK506, the nitrosyl-Hb level in DSG-treated graft was much higher than that in FK506-treated graft. There was no significant difference in survival time between DSG-treated and FK506-treated recipients. In conclusion, DSG and FK506 have a different effect on NO production in allografted liver with ongoing rejection, and circulating nitrite and /nitrate levels do not reflect the local levels of NO in the graft.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001470050233
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An improved method for the purification of stellate cells from rat liver with Dichloromethylene Diphosphate (CL2MDP) (1999)
    Springer
    Methods in cell science 21 (1999), S. 19-24 
    Details
    ISSN: 1573-0603
    Keywords: Dichloromethylene diphosphate ; Hepatic stellate cell isolation ; Liposome ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hepatic perisinusoidal cell population consists of hepatic stellate cells, Kupffer cells, endothelial cells, and Pit cells. These cells are isolated by enzymic digestion and purified by density gradient centrifugation. With isolation of stellate cells, conventional method is unable to eliminate the contamination of Kupffer cells because the densities of these two cells are similar. We report here an improved method for isolation of highly purified hepatic stellate cells, using dichloromethylene diphosphate (CL2MDP), which has selective cytotoxicity of Kupffer cells. Three days after the single intravenous administration of liposome-encapsulated CL2MDP, the Kupffer cells disappeared almost completely from the liver. Following Percoll density gradient centrifugation, the purity of the hepatic stellate cells exceeded 98% without any contamination of the Kupffer cells. Kupffer cells are reported to affect the physiological functions of stellate cells. The availability of highly purified stellate cells will facilitate the investigation of their functions in primary culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009872219428
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Gastric cancer recognized by metastasis to the ureter (2000)
    Springer
    Gastric cancer 3 (2000), S. 102-105 
    Details
    ISSN: 1436-3305
    Keywords: Key words Gastric cancer ; Ureteral metastasis ; Hydronephrosis ; Hydroureter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although ureteral obstruction is rarely noted in patients with gastric cancer at an advanced stage or at autopsy, the condition caused by authentic ureteral metastasis of gastric cancer is extremely rare. We experienced a case of gastric cancer in a 51-year-old woman who showed bilateral ureteral metastasis. The patient initially complained of right flank pain, caused by right ureteral obstruction, and was referred to our hospital, where she underwent a right nephroureterectomy, with suspicion of primary ureteral neoplasm. Histopathological examination of the resected specimen showed that metastatic growth of adenocarcinoma in the ureteral wall had caused the obstruction, and the subsequent extensive search for the primary lesion revealed asymptomatic gastric cancer. Soon after the nephroureterectomy, the patient developed left hydronephrosis, possibly caused by left ureteral metastasis, and a left percutaneous nephrostomy was performed. She then received chemotherapeutic reagents. However, she finally developed peritoneal carcinomatosis, and died of the disease about 1 year after the onset of the disease. In this report, we also review true ureteral metastasis from the stomach, and discuss the clinicopathologic features.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00011693
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    Paper (German National Licenses)
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