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  • 1
    Electronic Resource
    Electronic Resource
    Temperature-sensitive DNA repair mutations in the cellular slime mould Dictyostelium discoideum (1981)
    Springer
    Current genetics 3 (1981), S. 167-171 
    Details
    ISSN: 1432-0983
    Keywords: Dictyostelium discoideum ; DNA repair mutations ; Temperature-sensitive DNA repair ; DNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nonallelic radB13 and radG5 DNA repair mutations lower the plating efficiency at 26.5 °C of unirradiated D. discoideum cells. Revertants selected on the basis of resistance to gamma rays or UV from strains bearing either the radB13 or radG5 mutations are no longer temperature-sensitive at 26.5 °C. Unlike the wild-type and radiation-resistant revertants, survival following UV irradiation is lower at 24.5 °C than at 17.0 °C or 21.0 °C in strains carrying either the radB13 or radG5 mutation. We conclude that the gene products of the radB and radG loci probably affect normal cell growth by affecting DNA metabolism. Seven radiation-sensitive mutations that effect six loci other than radB and radG do not have temperature-sensitive phenotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429818
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The replication origin position and its relationship to a negative trans-acting transcription regulator encoded by Dictyostelium discoideum nuclear plasmid Ddp1 (1995)
    Springer
    Current genetics 27 (1995), S. 479-484 
    Details
    ISSN: 1432-0983
    Keywords: Dictyostelium discoideum ; Negative transcription regulator ; Nuclear plasmid ; Replication origin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The replication origin of the Dictyostelium discoideum plasmid Ddp1 was localized to a 543-bp region. This includes most of the AT-rich intergenic region between the G1 and G5/D6 genes containing both of their promoters and multiple copies of a TTTTGACT repeat. The G5/D6 gene, which lies adjacent to, and partially overlaps, the 543-bp origin region, encodes a trans-acting factor that negatively regulates transcription of the G4/D5 gene. Inactivation of the G5/D6 gene led to expression of a transcript (G6) 0.2 kb larger than the D5 transcript from the G4/D5 gene in vegetative and developing cells. The G5/D6 gene also regulates transcription of the G1, G2/G3/D4 and G5/D6 genes either alone or in concert with other Ddp1 gene products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00311219
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  • 3
    Electronic Resource
    Electronic Resource
    Chromosomal mapping of tRNA genes from Dictyostelium discoideum (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 176-187 
    Details
    ISSN: 1617-4623
    Keywords: Dictyostelium discoideum ; Restriction fragment length polymorphism ; tRNA genes ; Chromosomal organization ; Milti-copy genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Different wild-type isolates of Dictyostelium discoideum exhibit extensive polymorphism in the length of restriction fragments carrying tRNA genes. These size differences were used to study the organisation of two tRNA gene families which encode a tRNAVal(GUU) and a tRNAVal(GUA) gene. The method used involved a combination of classitics. The tRNA genes were mapped to specific linkage groups (chromosomes) by correlating the presence of polymorphic DNA bands that hybridized with the tRNA gene probes with the presence of genetic markers for those linkage groups. These analyses established that both of the tRNA gene families are dispersed among sites on several of the chromosomes. Information of nine tRNAVal(GUU) genes from the wild-type isolate NC4 was obtained: three map to linkage group I (C, E, F,), two map to linkage group II (D, I), one maps to linkage group IV (G), one, which corresponds to the cloned gene, maps to either linkage group III or VI (B), and two map to one of linkage groups III, VI or VIII (A, H). Six tRNAVal(GUA) genes from the NC4 isolate were mapped; one to linkage group I (D), two to linkage group III, VI or VII (B, C) and three to linkage group VII or III (A, E, F).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331507
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  • 4
    Electronic Resource
    Electronic Resource
    Linkage analysis of the myosin heavy chain gene in Dictyostelium discoideum using a mutation generated by homologous recombination (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 498-502 
    Details
    ISSN: 1617-4623
    Keywords: Dictyostelium discoideum ; Myosin ; Linkage analysis ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A mutation (mhcA1 in strain HMM) created by insertional gene inactivation was used to map the Dictyostelium discoideum myosin heavy chain gene (mhcA) to linkage group IV. Three phenotypic traits associated with this mutation (slow colony growth, inability of the mutant to develop past aggregation, and the presence of five to ten integrated vector copies) cosegregated as expected for the consequences of a single insertional event. This linkage was confirmed using a restriction fragment length polymorphism. The mhcA1 mutation was recessive to wild type and was nonallelic with mutations at the following loci on linkage group IV: aggJ, aggL, couH, minA, phgB and tsgB. This work demonstrates the ability to apply standard techniques developed for D. discoideum parasexual genetic analyses to mutants generated by transformation, which is of particular relevance to analysis of genes for which no classical mutations or restriction fragment length polymorphisms are available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334396
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  • 5
    Electronic Resource
    Electronic Resource
    Gene amplification associated with the dominant cob-354 cobalt resistance trait in Dictyostelium discoideum (1989)
    Springer
    Molecular genetics and genomics 220 (1989), S. 25-32 
    Details
    ISSN: 1617-4623
    Keywords: Dictyostelium discoideum ; Cobalt resistance ; Gene amplification ; Extrachromosomal ; Developmental defect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA amplification is correlated with the dominant, unstable cob-354 cobalt resistance trait in the cellular slime mold, Dictyostelium discoideum. The amplified DNA is present as about 50 copies of an extrachromosomal element. Cells grown under nonselective conditions in the absence of cobalt ions lose both the cobalt resistance trait and all extrachromosomal copies of the amplified DNA. The amplified DNA is transferrable to new genetic backgrounds by parasexual genetic crosses. These results explain the inability to map the cob-354 trait to a linkage group. The chromosomal origin of the amplified DNA is group III or VI. Thus the resistance trait appears to be independent of the previously known cobalt resistance locus, cobA, which maps to group VII. A developmental defect involving the production of multiply-tipped aggregates that do not complete fruiting body formation also is correlated with the presence of the amplified DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260851
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  • 6
    Electronic Resource
    Electronic Resource
    Mutations specific to spore maturation in the asexual fruiting body of dicytostelium discoideum (1979)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 1 (1979), S. 355-362 
    Details
    ISSN: 0192-253X
    Keywords: Dictyostelium discoideum ; spore maturation ; spore specific mutations ; cell patterning ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Three mutations affecting spore maturation in the asexual fruiting body of Dictyostelium discoideum are assigned to a new locus, sprJ, on linkage group IV. Strains carrying mutations at the sprJ locus do not form mature spores, yet the cell patterning (spore, stalk and disc cell ratios) is apparently normal. These mutations will be useful to delineate branch points between the cell patterning and spore maturation pathways. There are some unusual features of the sprJ-containing mutants. In particular each of the parent strains of the three mutants has incomplete spore maturation as determined by colony-forming ability after heat shocking at 45°C. A mutation allowing growth in the presence of benlate (600 μg/ml), benA351, is mapped to linkage group I.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020010408
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