ISSN:
1432-0568
Keywords:
Key words Microscopy
;
Digital film scanner
;
Image processing
;
Bright field
;
Multimedia
;
Medical education
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Low-power bright field photomicrographs often suffer from insufficient sharpness, uneven illumination, and colour hues. Using a film scanner, commercially available and designed for digitizing 35-mm transparencies, we directly scanned microscopic slides that carried dye-labelled and stained sections. The digital images covered a field of up to 24×36 mm and revealed excellent sharpness, absolutely even illumination and superior colour reproduction as compared to conventional photomicrographs taken with binoculars, macro lenses, or microscopes. As the method requires neither specialized instrumentation nor expert knowledge of photomicrographic techniques, it reduces costs and saves time. The high-quality digital survey micrographs can easily be used for image processing, image analysis and morphometry. Thus, this new method is valuable not only for pathology, embryology, histochemistry, and the neurosciences, but also for the exchange of low-power micrographs via the internet and for computer media that are increasingly used in medical education.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004290050194
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