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  • Dihydrorhodamine 123  (1)
  • Hip replacement  (1)
  • RAPD  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry (1991)
    Springer
    European journal of pediatrics 150 (1991), S. 161-165 
    Details
    ISSN: 1432-1076
    Keywords: Chronic granulomatous disease ; Dihydrorhodamine 123 ; Diagnosis ; Inheritance ; Flow microcytofluorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Dihydrorhodamine 123 (DHR) attached to membranes of granulocytes (PMN) and monocytes is caused to fluoresce by reactive oxygen intermediates (ROI) indicating the ability of phagocytes to produce these microbicide metabolites in a flow microcytofluorimeter. Whole blood samples from five boys with known chronic granulomatous disease (CGD) and from their mothers (and from one father and one grandmother), were examined following erythrocyte lysis in order to test this new method. An incubation period of 10 min with phorbol-myristate-acetate, followed by another 15 min incubation period with DHR before flow microcytofluorimetric analysis of 5 or 10×103 phagocytes, was sufficient to obtain the following results. PMN and monocytes from four patients with CGD could clearly not produce any ROI whereas cells from one patient displayed decreased activity in ROI production as compared to cells from a healthy donor. The X-linked mode of inheritance was detected in six carriers by the presence of two different cell populations (one normal ROI-producing and one negative or less active population). All the phagocytes from one mother produced ROI in normal amounts suggesting an autosomal mode of inheritance. All in all, the method presented provides a fast and most simple tool to diagnose CGD, to determine a decrease or total lack of ROI production and to establish the mode of inheritance of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01963557
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories (1997)
    Springer
    Molecular breeding 3 (1997), S. 381-390 
    Details
    ISSN: 1572-9788
    Keywords: DNA markers ; RAPD ; AFLP ; SSR ; microsatellite ; network ; reproducibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be exchanged between laboratories, which in turn requires that they can be standardised to yield reproducible results, so that direct collation and comparison of the data are possible. This article describes a network experiment involving several European laboratories, in which the reproducibility of three popular molecular marker techniques was examined: random-amplified fragment length polymorphism (RAPD), amplified fragment length polymorphism (AFLP) and sequence-tagged microsatellites (SSR). For each technique, an optimal system was chosen, which had been standardised and routinely used by one laboratory. This system (genetic screening package) was distributed to different participating laboratories in the network and the results obtained compared with those of the original sender. Different experiences were gained in this exchange experiment with the different techniques. RAPDs proved difficult to reproduce. For AFLPs, a single-band difference was observed in one track, whilst SSR alleles were amplified by all laboratories, but small differences in their sizing were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009612517139
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Unterschiedliche Wirksamkeit von zwei Präparaten mit niedermolekularem Heparin bei Patienten mit elektivem Hüftgelenksersatz (1991)
    Springer
    Langenbeck's archives of surgery 376 (1991), S. 147-151 
    Details
    ISSN: 1435-2451
    Keywords: Heparin ; Low-molecular weight ; Hip replacement ; Clinical study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In einer offen-randomisierten, dreiarmigen Studie bei 105 Patienten mit elektivem Hüftge-lenksersatz wurde die Wirksamkeit and Verträglichkeit eines niedermolekularen Heparins, einer Kombination eines anderen niedermolekularen Heparins mit Dihydroergotamin und eines unfraktionierten Heparins verglichen. Die Wirksamkeit wurde mittels Radiofibrinogentest während der ersten 7 postoperativen Tage sowie mittels beidseitiger Phlebographie am 14./15. postoperativen Tag überprüft. Bei gleichguter Verträglichkeit der 3 Prophylaxeschemata ergab sich eine deutliche Über-legenheit der Kombinationsprophylaxe gegenüber dem reinen niedermolekularen Heparin. Während die Thromboserate unter der Kombinationsprophylaxe bei 22,9% lag, betrug sic unter niedermolekularem Heparin allein 50,0% (2 p=0,03) and fur unfraktioniertes Heparin 30,6%.
    Notes: Summary In an open randomised trial 3 regimen of prophylaxis for deep vein thrombosis were compared in 105 patients undergoing elective hip arthroplasty. One part of the patients received one injection daily of a low molecular weight heparin, another part was treated with one injection of a combination containing a different type of low molecular weight heparin + dihydroergotamine per day and a third part of the patients received 3 applications of 5000 IU unfractionated heparin per day. The frequency of DVT determined by the 125iodine-labelled fibrinogen uptake test and phlebography was 22.9% in patients receiving the combination and 50.0% in those receiving the low-molecular weight heparin alone. The difference was statistically significant with 2 p = 0.03. The incidence of DVT in patients receiving unfractionated heparin was 30.6%. No difference in the development of bleeding complications was found between the three treatment groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00250339
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    Paper (German National Licenses)
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