ISSN:
1432-0738
Keywords:
Carcinogen-protein adducts
;
Tobacco-specific N-nitrosamines
;
Dosimeter of carcinogen exposure
;
Immunoassay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Polyclonal antibodies recognizing the pyridyloxobutyl (POB) moiety of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) were produced in rabbits immunized either with POB-bovine albumin or POB-Sepharose. The POB intermediates necessary to modify the protein were generated by alkaline (pH 9.0) treatment of the synthetic precursor 4-(carbethoxynitrosamino)-1-(3-pyridyl)-1-butanone. In a competitive enzyme linked immunoabsorbent assay (ELISA), 70 pmole NNK inhibited 50% of the binding of the anti-POB antibodies to POB-protein absorbed on microtiterplates. This 50% inhibition varied from 70 pmole to 200 nmole using a series of NNK analogues, depending on the integrity of the POB moiety. Immunological techniques initiated in this study detect NNK-protein conjugates or measure the quantity of POB groups liberated upon alkaline or acid treatment of NNK modified protein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01973456
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