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Serum levels of prolactin during the ovarian cycle, pregnancy, and lactation in the common marmoset (Callithrix jacchus) (1995)

Moro, Makoto ; Torii, Ryuzo ; Koizumi, Hitoshi ; [et al.]

Springer

Primates 36 (1995), S. 249-257

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ISSN: 0032-8332

Keywords: Common marmoset ; Prolactin ; Ovarian cycle ; Pregnancy ; Lactation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A homologous double-antibody radioimmunoassay developed for humans was used to measure serum prolactin, progesterone, and estradiol in common marmosets. In the ovarian cycle of common marmosets, serum progesterone began to increase after an estradiol surge, attained a peak level, and then declined before the ensuing pre-ovulatory rise in estradiol. During the luteal phase, the change in serum concentrations of estradiol was synchronized with that of progesterone. During the ovarian cycle there was no consistent change in serum prolactin concentrations. During the last 75 days of pregnancy the prolactin level was higher than during the ovarian cycle and the first 70 days of pregnancy. Moreover, during lactation, mothers with suckling twin infants had a higher prolactin level than during the final stage of pregnancy.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02381350

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Capillary supercritical fluid chromatography with flame photometric detection using a large bore column SFC pumping system (1991)

Koizumi, Hitoshi ; Suzuki, Yoshihito

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 14 (1991), S. 257-261

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ISSN: 0935-6304

Keywords: Capillary supercritical fluid chromatography ; Large bore column SFC pumping system ; Double-stage flow splitter ; Flame photometric detection ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A commercially available instrument with an SFC pumping system suitable for wide bore columns (4.6 mm i.d.) has been modified for capillary supercritical fluid chromatography (CSFC) by incorporating a double-stage flow splitter. The first flow splitter was installed in front of the sample injection valve in order to avoid a high solute split ratio. The second splitter was mounted in the column oven so that the injected sample (0.2 μL) would be split to the capillary column. In order to perform pressure programmed elution, a pressure regulating system equipped with a gradient programmer has been used. Flame photometric detection was optimized for the analysis of organosulfur compounds by CSFC. In this study, detection limits were found to be 6-14 ng and the experimentally determined exponent (n value) varied from 1.721 to 1.984 depending on the compounds tested. Sulfur- and phosphorus-containing thermally labile pesticides can be chromatographed and selectively detected by using CSFC/FPD in either sulfur- or phosphorus mode, respectively.

Additional Material: 5 Ill.