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Comparison of alterations of chromosome 17 in carcinoma of the ovary and of the breast (1999)

Caduff, R. F. ; Svoboda-Newman, Suzette M. ; Ferguson, Amy W. ; [et al.]

Springer

Virchows Archiv 434 (1999), S. 517-522

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ISSN: 1432-2307

Keywords: Key words Ovary ; Breast ; Carcinoma ; Loss of heterozygosity ; Chromosome 17

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Breast and ovarian carcinomas share a region of allelic loss on chromosome 17q25, suggesting that these tumours may arise by similar molecular pathways. We analysed paraffin-embedded tissues from 84 sporadic ovarian carcinomas and 42 sporadic infiltrating ductal carcinomas of the breast for abnormalities on chromosome 17. Loss of heterozygosity (LOH) of at least one informative marker on 17q was identified in 49 of 82 (60%) ovarian carcinomas, as against only 6 of 40 (15%) informative breast carcinomas (P〈0.0001). In ovarian carcinoma, LOH was most commonly observed for GH on 17q23 (56%), and was also frequently observed at 17q21 (46%). In contrast, LOH of D17S1330/CTT16 on 17q25 was observed in only 19% of ovarian tumours. LOH in breast carcinomas was most frequently observed at 17q21 (16%), less frequently at 17q23 (7%) and not identified at all at 17q25 in any breast cancers. Immunohistochemical analysis demonstrated overexpression of the p53 gene product in 38 of 84 (45%) ovarian carcinomas, as against 10 of 42 (24%) breast carcinomas (P=0.0195). p53 immunoreactivity was significantly associated with LOH in ovarian and breast cancers. Immunohistochemical expression of HER2/neu was observed in 6 of 84 (7%) ovarian and 3 of 42 (7%) breast carcinomas. There was no relationship between HER2/neu immunoreactivity and LOH. Although sporadic carcinomas of breast and ovary share some regions of allelic loss on chromosome 17q, differences in other alterations on this chromosome suggest divergent pathways of tumour development.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004280050377

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Trace-level determination of aromatic sulfonates in water by on-line ion-pair extraction/ion-pair chromatography and their behavior in the aquatic environment (1995)

Lange, Frank Thomas ; Wenz, Michael ; Brauch, Heinz-Jürgen

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 18 (1995), S. 243-252

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ISSN: 0935-6304

Keywords: Ion-pair extraction ; Ion-pair chromatography ; On-line combination ; Aromatic sulfonates ; Fate studies ; Waste water ; River water ; Drinking water production ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A new HPLC method for the fully automatic determination of aromatic sulfonates in aqueous samples is presented. The analytical procedure consists of an on-line combination of ion-pair extraction (IPE) and ion-pair chromatography (IPC), both using RP-C18 solid-phase material and a tetrabutylammonium salt as ion pairing reagent. Experimental details and performance data are given. This method is suited for the trace-level determination of a wide variety of benzene, naphthalene, anthraquinone and stilbene sulfonates. Detection limits for surface water using a diode-array detector are in the sub-ppb range. For naphthalene sulfonates a very good selectivity and minimal detectable limits of 0.02 μg/L or even lower can be achieved. So far, this method has been successfully applied to waste water, river water, bank filtrate, and water from different steps of drinking water production. The fate of several aromatic sulfonates has been studied beginning at the effluents of industrial waste water treatment plants and ending after activated carbon filtration in a water works. Napthalene-1,5-disulfonate (NDS, Armstrong acid) and cis-4,4′-dinitrostilbene-2,2′-disulfonate (DNS) appear in the raw water of the investigated water works and therefore have to be termed as relevant to water works. In contrast to other disulfonates NDS is extremely stable to biodegradation and ozonation and it is even desorbed from a highly loaded activated carbon filter.

Additional Material: 9 Ill.