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  • 1
    Digitale Medien
    Digitale Medien
    Isolation and characterization of pteridines from heads of Drosophila melanogaster by a modified thin-layer chromatography procedure (1977)
    Springer
    Biochemical genetics 15 (1977), S. 307-319 
    Details
    ISSN: 1573-4927
    Schlagwort(e): pteridines ; Drosophila ; thin-layer chromatography ; eye pigments
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract An improved thin-layer chromatography technique is described for the separation of fluorescent compounds found in extracts of heads of Drosophila melanogaster. Eighteen to twenty fluorescent spots are resolved, two of which are xanthurenic acid and 3-hydroxykynurenine, and the remaining spots are presumably pteridines. Of these, nine have been identified and quantitated directly on the chromatograms with a fluorometer. One of the spots present on the chromatogram apparently has not been described previous to this work. Characteristics of this substance, termed “quench spot,” are presented, several of which indicate that it may be a pteridine or pteridine derivative.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00484462
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Mechanism of suppression in Drosophila. V. Localization of the purple mutant of Drosophila melanogaster in the pteridine biosynthetic pathway (1977)
    Springer
    Biochemical genetics 15 (1977), S. 321-332 
    Details
    ISSN: 1573-4927
    Schlagwort(e): pteridines ; Drosophila ; suppression ; eye color mutants ; GTP ; development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The suppressible eye color mutant purple (pr) of Drosophila melanogaster is known to be unable to synthesize a wild-type complement of pteridine eye pigments. This study measures the reduced levels of drosopterins, sepiapterin, and an unidentified presumed pteridine in pr and pr bw. Pteridine analyses in double mutants combining pr with one of three other eye color mutants sepia, Henna-recessive3, and prune2, suggest that the metabolic block in pr occurs prior to sepiapterin biosynthesis. Measurements of GTP and GTP cyclohydrolase in pr showed wild-type levels and indicate the metabolic block in pr to be at one of the steps converting dihydroneopterin triphosphate to sepiapterin. Quantitation of pteridines in suppressed purple [su(s) 2; pr and pr; su(pr) e3] shows restoration of pteridines to wild-type or nearly wild-type levels.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00484463
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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