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  • 1
    Electronic Resource
    Electronic Resource
    Spectral and photophysical studies of thiazine dyes in triton X-100 (1988)
    Springer
    Colloid & polymer science 266 (1988), S. 672-676 
    Details
    ISSN: 1435-1536
    Keywords: Dye-surfactant ; thiazine dyes ; charge-transfer interaction ; thermodynamics ; photogalvanic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The absorption spectra of several thiazine dyes such as thionine, azure A, azure B, azure C and methylene blue in aqueous solution of Triton X-100 show that dyes as electron acceptors form 1∶1 charge-transfer (CT) or electron-donor-acceptor (EDA) complexes with Triton X-100, which acts as an electron donor. From the thermodynamic and spectrophotometric properties of these complexes, the abilities of dyes to accept an electron are in the order: azure C 〉 thionine 〉 azure A 〉 azure B 〉 methylene blue. The photogalvanic effect in the aqueous solution dye-surfactant has been studied. Generation of photovoltage supports a CT or EDA interaction between thiazine dyes and Triton X-100. There is a good correlation among the photophysical (photovoltage), spectral and thermodynamic properties of these complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01411509
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of genomic DNA from the earthworm speciesEisenia fetida (1995)
    Springer
    Molecular and cellular biochemistry 142 (1995), S. 19-23 
    Details
    ISSN: 1573-4919
    Keywords: DNA extraction ; earthworms ; Eisenia fetida ; 32P-postlabelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Our interest in detecting genotoxic exposure in earthworms led us to isolate high quality DNA from theEisenia fetida species. For that, we compared a modification of the conventional phenol-chloroform extraction procedure, usually refered to as the Maniatis procedure, to two commercially available kits reportedly eliminating multiple partitions in phenol and chloroform, namely the Qiagen and Nucleon protocols. From the 260 nm optical density values, the commercial kits extracts hinted toward higher DNA recovery with those procedures. However, the 260/280 nm ratios indicated that the quality of the DNA isolated with the modified Maniatis procedure was purer than that isolated with the commercial kits, the latter being most probably contaminated by proteins and/or RNA. The Maniatis procedure was slightly modified by the introduction of a potassium acetate step for protein precipitation and by shortening the proteinase K treatment from 12–18 h to only 2 h. The higher quality of the DNA isolated by phenol-chloroform extraction was confirmed by quantification with the fluorescent 3,5-diaminobenzoic acid assay. Preliminary results suggest that the modified Maniatis procedure herein described is not only applicable for DNA adducts studies using32P-postlabelling techniques but is also suitable for DNA extraction from other earthworm species such asLumbricus terrestris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00928909
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    Paper (German National Licenses)
    Fulltext
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