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  • 1
    ISSN: 1432-203X
    Keywords: Genetically engineered resistance ; segregation ratios ; potato leafroll luteovirus ; coat protein ; transcript RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The coat protein (CP) gene of potato leafroll luteovims (PLRV) was inserted into tobacco (Nicotiana tabacum) using disarmed Agrobacterium tumefaciens (LBA4404) containing a binary expression vector. PLRV CP gene transcript was detected in transgenic plants but its abundance differed between transformed lines. CP was not detected in virus-free transgenic plants. The segregation of kanamycin resistance in S1 seedling progenies (obtained by selfing transformed plants) indicated that multiple (up to five) integration events involving vector T-DNA had occurred in most transformants. However, the amount of detectable CP transcript was not related to the neomycin phosphotransferase II gene copy number. Multiplication of PLRV in mature transgenic plants was diminished by up to 6-fold; the greatest diminution was in those transformed lines in which most CP gene transcript was detected. However, S1 progeny seedlings of transgenic plants were no more resistant to infection, following inoculation with viruliferous aphids, than seedlings of non-transformed control plants.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    International urogynecology journal 2 (1991), S. 228-231 
    ISSN: 1433-3023
    Keywords: Dysfunctional ; Girls ; Incontinence ; Unstable ; Urodynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The value of a simplified urodynamic evaluation was assessed in girls with diurnal and/or nocturnal enuresis who had no overt obstruction or neuropathy. The 111 girls evaluated were divided into three groups. Forty-three had normal findings, that is to say they were stable during filling and voiding was coordinated and complete. Fifty-three had unstable bladders during filling but voiding was normal and coordinated. Fifteen had dyssynergic voiding and an increased residual urine. The urodynamic findings were helpful in distinguishing these groups with their different management options. The data also indicated that they are two distinct groups, one with instability alone and one with dysfunctional voiding with or without instability. Although infection and reflux are common with instability alone, the more devasting urinary tract changes, in particular heavy trabeculation and elongation of the bladder, occur with dysfunctional voiding.
    Type of Medium: Electronic Resource
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