Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Cytoplasmic calcium levels in protoplasts from the cap and elongation zone of maize roots (1991)
    Springer
    Protoplasma 163 (1991), S. 181-188 
    Details
    ISSN: 1615-6102
    Keywords: Cytoplasmic calcium ; Fura ; Indo ; Protoplasts ; Gravitropism ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calcium has been implicated as a key component in the signal transduction process of root gravitropism. We measured cytoplasmic free calcium in protoplasts isolated from the elongation zone and cap of primary roots of light-grown, vertically oriented seedlings ofZea mays L. Protoplasts were loaded with the pentapotassium salts of fura-2 and indo-1 by incubation in acidic solutions of these calcium indicators. Loading increased with decreasing pH but the pH dependence was stronger for indo-1 than for fura-2. In the case of fura-2, loading was enhanced only at the lowest pH (4.5) tested. Dyes loaded in this manner were distributed predominantly in the cytoplasm as indicated by fluorescence patterns. As an alternative method of loading, protoplasts were incubated with the acetoxymethylesters of fura-2 and indo-1. Protoplasts loaded by this method exhibited fluorescence both in the cytoplasm and in association with various organelles. Cytoplasmic calcium levels measured using spectrofluorometry, were found to be 160±40 nM and 257±27 nM, respectively, in populations of protoplasts from the root cap and elongation zone. Cytoplasmic free calcium did not increase upon addition of calcium to the incubation medium, indicating that the passive permeability to calcium was low.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01323342
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Response of ethylene and chlorophyll in two eucalyptus clones during drought (1995)
    Springer
    New forests 9 (1995), S. 197-204 
    Details
    ISSN: 1573-5095
    Keywords: water stress ; transpiration ; chlorophyll ; ethylene ; Eucalyptus grandis ; E. camaldulensis ; E. robusta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A study was conducted with seven-month-old plantlets, of the clone 2814 of Eucalyptus grandis × E. camaldulensis Dehnh. hybrid and of the clone 2798 of E. grandis × E. robusta Sm., subjected to sublethal water stress. During the imposed drought, leaf water potentials and transpiration rates decreased, while ethylene production and leaf chlorophyll concentration increased to a maximum during the onset of water stress, and then declined. Stomatal closure coincided with or preceded maximum ethylene production. A saturation type relationship between ethylene production and chlorophyll concentration was observed. Genotypic differences in the response to water stress occurred between clones with clone 2814 appearing to be a drought avoider and clone 2798 a drought tolerator.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035487
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...