ISSN:
1612-1112
Keywords:
Immunoaffinity chromatography
;
Human epidermal growth factor (hEGF)
;
Immobilization of antibody
;
Pore size of diol silica
;
Optimum coupling conditions
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The preparation of support-coupled antibodies for high-performance immunoaffinity chromatography has been demonstrated. Diol silica had the least non-specific adsorption of all supports investigated. The optimum activation conditions for diol silica with 1,1′-carbonyl-diimidazole and the optimum coupling conditions for anti-human epidermal growth factor antibody as a function of salt concentration, pH and ligand concentration were selected. The pore size of the diol silica was an important factor in achieving good results. Diol silica with 500Å pore size had high loading capacity against the antigen. The use of F(ab′)2 or Fab as a ligand showed almost the same chromatographic characteristics as IgG.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02258980
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