ISSN:
1573-4986
Keywords:
ELISA
;
urinary tetrasaccharide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The tetrasaccharide, Glcα1-6Glcα1-4Glcα1-4Glc, denoted (Glc)4, is a limit dextrin formed by amylolytic degradation of glycogen. In order to evaluate the possible clinical importance of (Glc)4 excretion as an indicator of certain physiological and pathological conditions, we have developed a new rapid and inexpensive immunoassay using a monoclonal antibody raised against (Glc)4 glycosidically-linked to a carrier protein. As the antibody is highly specific, it can be used to measure native (Glc)4 directly, without the chemical reduction step required in previous methods. A new type of non-equilibrium ELISA inhibition test was developed based on the capacity of free (Glc)4 to decrease initial rates of antibody binding to (Glc)4-coated microtiter wells. The method is highly reproducible and is as sensitive and accurate as the gas chromatography method or radioimmunoassay used previously.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01108614
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