ISSN:
1433-8580
Keywords:
G-CSF
;
Monoclonal antibody
;
ELISA
;
CFU-GM
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary An IgG monoclonal antibody to recombinant human granulocyte colony-stimulating factor (G-CSF), designated HG1, was produced by fusion of immune mouse splenocytes with HAT-sensitive murine myeloma cells. This HG1 was capable of neutralizing the colony-stimulating activity of G-CSF in vitro, and it did not cross-react with human granulocyte/macrophage colony-stimulating factor (GM-CSF). An enzyme-linked immunosorbent assay (ELISA) for measurement of G-CSF was developed using HG1 and a polyclonal antibody against G-CSF raised in a rabbit. The data indicated that the ELISA was highly efficient and sensitive for the detection of as little as 50pg/ml of recombinant G-CSF. This assay system therefore warrants further attention.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01852164
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