ISSN:
1573-2568
Keywords:
ileal pouch-anal anastomosis
;
pouchitis
;
ulcerative colitis
;
familial adenomatous polyposis
;
shortchain fatty acids
;
bile acids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Construction of an ileal reservoir changes the fecal bacterial flora and the fecal composition of bile acids and short-chain fatty acids. We examined the relationships between pouch inflammation (pouchitis) and pouch content, as assessed by analysis of fecal bacteria, bile acids, and short chain fatty acids. Four groups were studied: ileal pouch-anal anastomosis (IPAA) for ulcerative colitis with pouchitis (N=10), IPAA without pouchitis (N=5), IPAA for familial adenomatous polyposis without pouchitis (N=5); and Brooke ileostomy for ulcerative colitis, which served as controls (N=5). Pouchitis was defined as ≥7 points on an 18-point pouchitis disease activity index. Aerobic and anaerobic bacteria were quantitatively cultured. Total aqueous-phase bile acids were measured by thin-layer chromatography and an enzymatic 3α-OH hydroxysteroid dehydrogenase method. Fecal short chain fatty acids were measured by gas liquid chromatography. All patients with an IPAA had higher ratios of anaerobes/aerobes and concentrations of anaerobic gram-negative rods than did patients with an ileostomy. There were no other differences between patient groups with respect to bacteria, aqueous-phase total bile acids, or fecal short-chain fatty acids. Fecal concentrations of bacteria, bile acids, and short-chain fatty acids were similar in patients with and without pouchitis, indicating that these factors can not be the sole cause of pouchitis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02285195
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