ISSN:
1573-7438
Keywords:
buffalo
;
culture
;
diagnosis
;
Ehrlichia bovis
;
monocytes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Monocytes from a buffalo were cultured in RPMI 1640 medium following separation of plasma by the erythrocyte sedimentation technique and subsequent separation of mononuclear cells by density gradient centrifugation. Growth of an organism considered to be Ehrlichia bovis was noticed in the cultured monocytes after 10 days. The inclusions were considered to be those of E. bovis from their morphology, staining characteristics and growth characteristics in culture, and by indirect immunofluorescence examination with an anti-E. canis serum. The utility of peripheral blood monocyte cultures opens the possibility of diagnosing the carrier status of ehrlichiosis in animals.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005270101276
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