ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
Electrochemical detection
;
Serum
;
Tetraiodothyronine
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Tetraiodo-L-thyronine (T4) in human serum was determined by HPLC using a nonphysiological internal standard. For sample preparation, a cation exchange resin, RP-chromatography on C-18 or phenylphase, and electrochemical detection (Metrohm 656 or BAS-LC 4 A) were employed. The mobile phase (0.8 ml/min) was 10 mM H3PO4/CH3OH (40/60 v/v) or 10 mM H3PO4/CH3CN (70/30 v/v, 1.5 ml/min), the voltage applied 800 mV. The coefficient of variation (day to day) was 6.8% (N = 40); concentrations of T4 found were 11 to 20% lower than those obtained by two different commercially available immunoassays.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240071004
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