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  • Electrophoretic karyotype  (2)
  • Heterokaryon incompatibility  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Mapping translocation breakpoints by orthogonal field agarose-gel electrophoresis (1996)
    Springer
    Current genetics 29 (1996), S. 301-305 
    Details
    ISSN: 1432-0983
    Keywords: Translocation breakpoint ; Neurospora crassa ; OFAGE ; Electrophoretic karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Orthogonal field agarose-gel electrophoresis (OFAGE) of chromosomes from translocation-bearing and normalNeurospora crassa strains was utilized, first, to recover cosmids from a translocated region, and second, to map translocation breakpoints. Surprisingly, the right breakpoints in two independently derived, interstitial translocations,T(II → III) AR18 andT(II → VI)P2869, are within about 5.6 kbp of each other suggesting that this region of linkage group (LG) II may be fragile or otherwise subject to chromosome breakage. Mapping translocation breakpoints through OFAGE, or other similar methods, should allow for DNA sequencing across breakpoints that are not associated with mutant phenotypes or that are not within walking distance of cloned markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221562
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Mapping translocation breakpoints by orthogonal field agarose-gel electrophoresis (1996)
    Springer
    Current genetics 29 (1996), S. 301-305 
    Details
    ISSN: 1432-0983
    Keywords: Key words Translocation breakpoint ; Neurospora crassa ; OFAGE ; Electrophoretic karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Orthogonal field agarose-gel electrophoresis (OFAGE) of chromosomes from translocation-bearing and normal Neurospora crassa strains was utilized, first, to recover cosmids from a translocated region, and second, to map translocation breakpoints. Surprisingly, the right breakpoints in two independently derived, interstitial translocations, T(II→III) AR18 and T(II→VI)P2869, are within about 5.6 kbp of each other suggesting that this region of linkage group (LG) II may be fragile or otherwise subject to chromosome breakage. Mapping translocation breakpoints through OFAGE, or other similar methods, should allow for DNA sequencing across breakpoints that are not associated with mutant phenotypes or that are not within walking distance of cloned markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050050
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The molecular nature of mutations in the mt A-1 gene of the Neurospora crassa A idiomorph and their relation to mating-type function (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 115-122 
    Details
    ISSN: 1617-4623
    Keywords: Key words Neurospora ; Mating type ; Mutations ; Heterokaryon incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The 293-amino acid mt A-1 ORF of the A mating-type idiomorph of Neurospora crassa is multifunctional. It confers A mating identity and is responsible for heterokaryon incompatibility. The goal of this study was to dissect the functional regions of mt A-1. New mutants of mt A-1 selected for loss of the incompatibility function were obtained. One new mutant, A m99 , was partially fertile as a maternal parent. This is the first time that fertility and incompatibility functions have been separated for the A idiomorph. In this mutant, the mt A-1 ORF is truncated after the first 85 amino acids, indicating that this N-terminal region is minimally sufficient for female fertility. A series of deletion constructs and frameshift alleles of mt A-1 was obtained and tested for male-mating activity and vegetative incompatibility in transformation experiments. These experiments showed that a region from position 1 to 111 is sufficient to confer incompatibility, while amino acids from position 1 to 227 are required for mating activity. A transcriptional analysis of mt A-1 showed that the mRNA is expressed both before and after fertilization. This, together with the phenotype of the A m99 mutant, suggests a post-fertilization function for mt A-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191831
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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