ISSN:
1432-2048
Keywords:
Key words: Brefeldin A
;
Daucus
;
Endomembrane syst-em
;
Protein secretion
;
Somatic embryogenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. Brefeldin A, an inhibitor of protein secretion, caused typical alterations to the endomembrane system with limited effects on viability when given to unorganized carrot cells growing in suspension. When given to the same cells during particular stages of embryogenesis, it caused similar endomembrane lesions and an almost complete arrest of the embryogenic process. Addition of conditioned medium containing extracellular secreted proteins to the embryos during treatment with Brefeldin A allowed acquisition of polarity and the continuation of a quasi-normal embryogenic process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004250050172
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