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  • 1
    Electronic Resource
    Electronic Resource
    Genetic transformation of Cymbidium orchid by particle bombardment (1999)
    Springer
    Plant cell reports 18 (1999), S. 978-984 
    Details
    ISSN: 1432-203X
    Keywords: Key words Orchid ; Particle bombardment ; PLB ; Transformation ; Key terminologyProtocorm A small storage organ formed from the germinating embryo ; possessing an apical meristem and a leaf primordium ; protocorm-like body (PLB) A somatic protocorm derived from in vitro culture of apical or axillary bud meristems ; primary PLBs PLBs induced by culturing apical meristem-tips aseptically ; secondary PLBs PLBs formed on the surface of a primary PLB in culture ; proliferate PLBs PLBs proliferating on the surface of either primary or secondary PLBs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH200) carrying the GUS-INT and NPTII genes flanked by tobacco matrix attachment regions was introduced into the meristematic cells of PLBs by particle acceleration. The transformed PLBs were proliferated and selected for kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was then induced from the kanamycin-resistant PLBs, and transgenic plantlets were produced. Both the kanamycin-resistant PLBs and regenerated shoots expressed the GUS-INT gene. The presence of the introduced gene in the transformed orchid plants was confirmed by PCR analysis, sequencing and Southern blot analysis of the PCR product. The recovered transgenic plants were established in soil and acclimatized in the greenhouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050694
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  • 2
    Electronic Resource
    Electronic Resource
    An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus) (2000)
    Springer
    Plant cell reports 19 (2000), S. 315-320 
    Details
    ISSN: 1432-203X
    Keywords: Key words Poplar ; Populus ; Aspen ; Cottonwood ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050019
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of cylindrical shells under patch loading (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Communications in Applied Numerical Methods 2 (1986), S. 571-585 
    Details
    ISSN: 0748-8025
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The object of this study is to predict the peak values of the stress resultants and couples in a relatively thick cylindrical shell under a concentrated local loading in order to guide the design of a large-scale reinforced concrete test specimen. Four finite element programs, SHORE-III, two versions of RONALD, and ANSYS, were employed here. The SHORE-III and RONALD analyses considered a complete cantilevered cylinder, while the ANSYS model treated a cylindrical panel free along the curved edge and constrained in various ways along the longitudinal edge. Comprehensive comparative results are provided for the linear elastic loading case, which indicate the magnitude and attenuation of concentrated loading effects on cylindrical shells and panels.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cnm.1630020605
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    Paper (German National Licenses)
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