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  • Enriched microsatellite library  (1)
  • Furobenzofuran  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Influence of trace elements and nitrogen sources on versicolorin production by a mutant strain of Aspergillus parasiticus (1979)
    Springer
    Mycopathologia 69 (1979), S. 161-166 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillus parasiticus ; Furobenzofuran ; Versicolorin A ; Versicolorin C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A mutant strain of Aspergillus parasiticus blocked in aflatoxin biosynthesis accumulates versicolorin A and versicolorin C. The effect of trace elements on the growth and versicolorin production by this strain was studied in a defined medium. The omission of manganese was slightly stimulatory to versicolorin production; when zinc was omitted from the medium, no detectable versicolorins were produced. Experiments on nitrogen sources in a highsucrose medium indicated that fourfold to fivefold increases in versicolorin yields could be obtained by substituting 3 ml/l corn steep liquor or 0.1 M NH4NO3 for the 0.023 M (NH4)2SO3 used previously as the nitrogen source in studies on versicolorin production by this strain. These improved yields will facilitate attempts to accumulate enough versicolorin A and versicolorin C for toxicity and carcinogenicity testing. Chromatographic profiles of mycelial extracts of cultures grown in a defined medium with 0.1 M NH4NO3 as the nitrogen source revealed 2 previously unrecognized compounds. The accumulation of these new metabolites in a mutant blocked in aflatoxin production may indicate that they are biosynthetically related to aflatoxin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00452829
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Abundance and polymorphism of microsatellite markers in the tea tree (Melaleuca alternifolia, Myrtaceae) (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1091-1098 
    Details
    ISSN: 1432-2242
    Keywords: Key words Simple sequence repeats ; Enriched microsatellite library ; Molecular marker ; Myrtaceae ; Melaleuca
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The sequencing of 831 clones from an enriched microsatellite library of Melaleuca alternifolia (Myrtaceae) yielded 715 inserts containing repeat motifs. The majority of these (98%) were dinucleotide repeats or trinucleotide repeats averaging 22 and 8 repeat motifs respectively. The AG/GA motif was the most common, accounting for 43% of all microsatellites. From a total of 139 primer pairs designed, 102 produced markers within the expected size range. The majority of these (93) were polymorphic. Primer pairs were tested on five selected M. alternifolia genotypes. Loci based on dinucleotide repeats detected on average a greater number of alleles (4.2) than those based on trinucleotide repeats (2.9). The loci described will provide a large pool of polymorphisms useful for population studies, genetic mapping, and possibly application in other Myrtaceae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051172
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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