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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 73 (1994), S. 183-186 
    ISSN: 1570-7458
    Keywords: plant-insect interactions ; antibiosis ; ovarian failure ; non host ; Glycine max ; Epilachna varivestis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 42 (1986), S. 19-26 
    ISSN: 1570-7458
    Keywords: Constitutive resistance ; soybean ; antifeedant ; Epilachna varivestis ; Mexican bean beetle ; herbivores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les paramètres de la résistance spontanée (c.a.d. non induite) du soja à E. varivestis ont été examinés sur des PI 227687 cultivés dans des conditions contrôlées et reproductibles d'un phytotron assurant une croissance et un développement optimaux. Les antiappétants de PI 227687 contre E. varivestris présentaient différentes solubilités dans l'hexane, l'acétate, d'éthyle et le méthanol à 100%. Les produits du métabolisme phénylpropanoïde des plantes, y compris les phytoalexines, sont fortement solubles dans un ou plusieurs de ces solvants. Les antiappétants sont restés dans le résidu aqueux après les extractions organiques séquentielles. Les PI 227687 sains, ainsi que les Davis susceptibles ont présenté les caractéristiques temporelles de l'activité tyrosine-amonia lyase. Les niveaux d'activité de ces deux enzymes sont très significativement corrélés avec le métabolisme phenylpropanoïde de la plante. Les teneurs en phénols totax de PI 227687 et Davis ne présentaient pas de différences. Les résultats semblent compatibles avec la présence des produits aglycônes du métabolisme phénylpropanoïde comme paramètres spontanés principaux de la résistance de PI 227687 à E. varivestis.
    Notes: Abstract Constitutive (i.e., preformed) parameters of Mexican bean beetle (MBB) resistance were demonstrated in PI 227687 soybeans grown under highly controlled and reproducible biotron environmental conditions which favored optimal plant growth and development. Constitutive antifeedants in PI 227687 against the beetle were variously soluble in hexane, ethyl acetate and 100% methanol. Antifeedants also remained in the aqueous residue after the sequential organic extractions. PI 227687 and MBB-susceptible ‘Davis’ soybean lines each showed characteristic temporal patterns of L-phenylalanine ammonia-lyase activity. Healthy PI 227687, but not healthy ‘Davis’, also showed a characteristic temporal pattern of L-tyrosine ammonia-lyase activity. Activities of the above two enzymes are positively correlated with plant phenylpropanoid metabolism. PI 227687 and ‘Davis’ leaves did not differ in content of total phenols. Products from phenylpropanoid metabolism apparently contribute to PI 227687 resistance to MBB feeding.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-1561
    Keywords: Allelochemicals ; isofiavonoids ; coumestrol ; antixenosis ; Epilachna varivestis ; Mexican bean beetle ; Coleoptera ; Coccinellidae ; Glycine max ; Phaseolus lunatus ; feeding bioassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The antixenosic properties of the isoflavonoid, coumestrol, were tested in dual-choice leaf disk bioassays with the Mexican bean beetle (Epilachna varivestis Mulsant).E. varivestis preferred the methanol-r,reated (solvent-control) disk when the coumestrol concentration was 1.8 or 0.9μ/leaf disk. No preference was observed between the coumestrol-treated and the solvent-control disks when the coumestrol concentration was higher, at 3.6, or lower, at 0.45μg/leaf disk. Coumestrol alone clearly is not responsible for the significant constitutive antixenosic properties of “Davis” soybeans,Glycine max (L.) Merrill, because the amount of coumestrol in these plants is significantly less than the minimum concentration which was antixenosic in this study. However, it might contribute to a constitutive antixenosis in “Davis” involving a profile of allelochemicals. A computer-aided densitometer, adapted to measure the leaf disk area, increased the resolution of the leaf area 250 (X)-fold as compared to the standard LI-COR leaf area meter.
    Type of Medium: Electronic Resource
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