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Digitale Medien

Method for generation of human B lymphoblastoid cell lines using Epstein-Barr virus (1995)

Lam, K. M. C. ; Crawford, D. H.

Springer

Methods in cell science 17 (1995), S. 67-74

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Details

ISSN: 1573-0603

Schlagwort(e): B lymphocytes ; Epstein-Barr virus ; Immortalization ; Lymphoblastoid cell line

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract A detailed methodology is described for the successful immortalization of B lymphocytes to give lymphoblastoid cell lines using Epstein-Barr (EB) virus. A virus stock is prepared from B95-8 or Akata cell line, which spontaneously release high titers of virus, and the immortalizing titer of the preparation determined. B lymphocytes from fresh or cryopreserved samples are separated from the whole cell population, infected with EB virus and cultured for 4 weeks for the establishment of a lymphoblastoid cell line. The presence of EB virus encoded proteins in the immortalized cell line can be detected by indirect immunofluorescent staining.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00986653

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