ISSN:
1573-5001
Keywords:
Ernst angle
;
HMQC
;
Screening (SAR)
;
Ligand binding
;
SH3
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The application of Ernst angle pulses in multidimensional NMR spectroscopy is theoretically and experimentally investigated. Theory shows that only for a few pulse sequences employed at high repetition rate, a remarkable gain in sensitivity is possible using Ernst angle pulses. As an example, a new variant of the heteronuclear multiple quantum coherence (HMQC) experiment, the fast-(1H,15N)-HMQC, is described. This sequence allows, with a 1 mM protein sample in H2O, the acquisition of a highly resolved two-dimensional (1H,15N) correlated spectrum within 37 s. The high efficiency of the fast-HMQC to detect ligand binding to a target protein is demonstrated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018361214472
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