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  • Escherichia coli  (4)
  • Glutamic acid decarboxylase  (2)
  • Layered manufacturing  (2)
  • Zea mays  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Overproduction, purification and characterization of M.HinfI methyltransferase and its deletion mutant
    Amsterdam : Elsevier
    Gene 113 (1992), S. 83-88 
    Details
    ISSN: 0378-1119
    Keywords: Escherichia coli ; Haemophilus influenzae ; overexpression ; recombinant DNA ; retroregulator stem-loop ; tac promoter
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(92)90672-C
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Overproduction, purification and characterization of M..HinfI methyltransferase and its deletion mutant
    Amsterdam : Elsevier
    Gene 121 (1992), S. 183 
    Details
    ISSN: 0378-1119
    Keywords: Escherichia coli ; Haemophilus influenzae ; overexpression ; recombinant DNA ; retroregulator stem-loop ; tac promoter
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(92)90181-N
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning, sequencing, overproduction, and purification of M.CviBI (GANTC) methyltransferase from Chlorella virus NC-1A
    Amsterdam : Elsevier
    Gene 126 (1993), S. 159 
    Details
    ISSN: 0378-1119
    Keywords: Escherichia coli ; gene cvibIM ; methylase ; recombinant DNA ; restriction endonuclease
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(93)90608-6
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning, sequencing, overproduction, and purification of M CviBI (GANTC) methyltransferase from Chlorella virus NC-1A
    Amsterdam : Elsevier
    Gene 121 (1992), S. 1-7 
    Details
    ISSN: 0378-1119
    Keywords: Escherichia coli ; gene cvibIM ; methylase ; recombinant DNA ; restriction endonuclease
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(92)90155-I
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A method for assessing geometrical errors in layered manufacturing. Part 2: Mathematical modelling and numerical evaluation (1998)
    Springer
    The international journal of advanced manufacturing technology 14 (1998), S. 644-650 
    Details
    ISSN: 1433-3015
    Keywords: Error interaction ; Error transfer ; Geometrical error ; Layered manufacturing ; Mathematical model ; Rapid prototyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Layered manufacturing based rapid prototyping processes are subjected to not only a staircase effect owing to the approximating process used, but also an accumulated error transfer between layers. Certain types of error such as surface tilting and layer thickness variations that occur in one layer can transfer to other layers above it. This paper describes a mathematical model based on the physical and geometrical models discussed in Part 1 of the paper. The model uses the matrix transformation method to analyse the effect of transformation of local errors to the multiple layer global errors. A data preparation error transformation matrix is used to describe the error interactions between layers during the data preparation stage. A disturbance error transformation matrix is used to describe the error interaction within each layer and error propagation between layers during the part building process. This model describes geometrical error analysis involving profiling error, layer inclination and layer thickness variations. Numerical evaluation of the model is carried out for a typical benchmark component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01192284
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  • 6
    Electronic Resource
    Electronic Resource
    Heterogeneity of islet pathology in two infants with recent onset diabetes mellitus (1995)
    Springer
    Virchows Archiv 425 (1995), S. 631-640 
    Details
    ISSN: 1432-2307
    Keywords: Recent-onset insulin dependent diabetes mellitus ; Insulitis ; Islet cell macrophages ; Glutamic acid decarboxylase ; Islet destruction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The mechanisms by which the beta cells of pancreatic islets are destroyed in insulin-dependent diabetes mellitus (IDDM) are poorly understood. In this report the pancreatic histo- and immunopathology of two children, both HLA-DR 3/4, DQ 2/8 positive and who both died from cerebral oedema within a day of clinical diagnosis of IDDM, were investigated. Patient 1, a 14-month-old girl, had a 4-week history of polydipsia and polyuria. Patient 2, a 3-year-old boy, had 2 days of illness. Both patients had a similarly severe loss of insulin cells but differed markedly as to the extent of lymphocytic islet infiltration (insulitis). Apart from insulitis, marked islet macrophage infiltration was demonstrated in both patients with the HAM-56 monoclonal antibody. Neither patient showed aberrant expression of HLA class II antigens on insulin-immunoreactive cells, but allele-specific HLA-DQ8 expression was evident on endothelial cells. Glutamic acid decarboxylase immunoreactivity was detected in both insulin- and glucagon-immunoreactive cells. It is concluded that the heterogeneity of islet pathology, especially insulitis, may reflect different dynamics and extent rather than different pathomechanisms of immune destruction of islets in IDDM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199353
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM (1994)
    Springer
    Diabetologia 37 (1994), S. 344-350 
    Details
    ISSN: 1432-0428
    Keywords: Glutamic acid decarboxylase ; receiver-operating characteristic plot ; diagnostic accuracy ; islet cell antibodies ; autoimmunity ; diabetes mellitus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Insulin-dependent diabetes mellitus (IDDM) is associated with autoreactivity against GAD but the diagnostic sensitivity (positivity in disease) and specificity (negativity in health) of isoform-specific GAD antibodies have yet to be defined in assay systems suitable for screening large number of samples. One set of IDDM patient (n=10) and control (n=50) standard sera were used to develop quantitative antibody assays with in vitro synthesized recombinant 35S-methionine-labelled GAD65 and GAD67, respectively, and protein A-Sepharose to separate free from antibody-bound ligand. Binding levels were not normally distributed (p〈0.0001) and therefore, the diagnostic accuracy of GAD antibodies was analysed by the ROC plots in population-based, consecutively-diagnosed, recent onset, 0–14 year-old patients (n=105), and matched, healthy control subjects (n=157). The ROC plots showed that the diagnostic sensitivity of GAD65 antibodies was 77% and the specificity 92% compared with 8% and 98%, respectively for GAD67 antibodies. In the IDDM sera, GAD65 and GAD67 antibodies were concordant in 7% (6 of 81) and GAD65 antibodies and ICA in 89% (72 of 81) without a correlation between the autoantibody levels. Autoantibodies to recombinant human islet GAD65 are specific and sensitive markers for childhood IDDM in this immunoassay with in vitro synthesized 35S-methioninelabelled recombinant GAD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408469
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of plant growth regulators on the antioxidant system in seedlings of two maize cultivars subjected to water stress (1998)
    Springer
    Plant growth regulation 25 (1998), S. 81-87 
    Details
    ISSN: 1573-5087
    Keywords: antioxidant system ; brassinolide ; drought stress ; methyl jasmonate ; uniconazole ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of brassinolide, uniconazole and methyl jasmonate on the antioxidant system were studied in seedlings of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of Zea mays L. When seedlings treated with the three regulators were subjected to water stress (−1.0 MPa PEG 6000 solution), the activities of superoxide dismutase, catalase and ascorbate peroxidase, as well as the ascorbic acid and total carotenoid contents, increased in the resistant cultivar, whereas the levels remained unaltered in the sensitive cultivar. The increased tolerance to drought stress induced by the growth regulators in the resistant cultivar seems to be due to the maintenance of increased antioxidant enzyme activity and antioxidant substance levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010774725695
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    A method for assessing geometrical errors in layered manufacturing. Part 1: Error interaction and transfer mechanisms (1998)
    Springer
    The international journal of advanced manufacturing technology 14 (1998), S. 637-643 
    Details
    ISSN: 1433-3015
    Keywords: Error assessment ; Error interaction ; Error transfer process ; Geometrical model ; Layered manufacturing ; Rapid prototyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Geometric accuracy of components is one of the most important quality characteristics in layered manufacturing processes on which most rapid prototyping (RP) techniques are based. Layered manufacturing is an approximate fabricating process in which the final geometric error of the physical part is affected, not only by the approximation technique used, but also by the fabrication process. Errors that occur in one layer could propagate and transfer to other layers causing an accumulated error effect in the process. In this paper, a concept of disturbance error is introduced to describe the effect of accumulated errors in the fabrication process. A physical model is presented to describe error interactions and error transfer mechanisms in the layered manufacturing process. A geometrical model is developed using surface approximation techniques to describe the relationships of the geometrical errors. It is shown that although the complexity of the part geometry is not directly related to the manufacturing process, it will affect the geometrical errors of the part produced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01192283
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  • 10
    Electronic Resource
    Electronic Resource
    Effects of plant growth regulators on the antioxidant system in callus of two maize cultivars subjected to water stress (1998)
    Springer
    Plant growth regulation 24 (1998), S. 55-66 
    Details
    ISSN: 1573-5087
    Keywords: antioxidant system ; brassinolide ; callus ; methyl jasmonate ; paraquat ; sodium benzoate ; uniconazole ; water stress ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of brassinolide, uniconazole and methyl jasmonate on several aspects of antioxidant defences, were studied in callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize. When regulator-treated calli were subjected to water stress with PEG for 24 h the activities of antioxidant enzymes, such as superoxide dismutase, catalase, ascorbate peroxidase, peroxidase and glutathione reductase, remained higher in callus of the drought-resistant than in callus of the drought-sensitive cultivar. Damage, as indicated by the levels of hydrogen peroxide and malondialdehyde, the reduction of ascorbate and carotenoids, and leakage of electrolytes from cells was apparent in callus of both cultivars as a consequence of the applied water stress. However, the damage was less marked in the drought-resistant cultivar. The regulator-treated callus of this cultivar also had a higher survival percentage than that of the drought-sensitive cultivar. The present results also compare the effects of growth regulators on antioxidant systems in callus tissue of different drought-resistant cultivars when exposed to paraquat and water stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005954532397
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