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  • Esophagus  (1)
  • Key words Malignant fibrous histiocytoma  (1)
  • 1
    ISSN: 1432-2307
    Schlagwort(e): Key words Malignant fibrous histiocytoma ; p53 ; mdm2 ; Prognosis and immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  TP53 and MDM2 genes and their protein expression were evaluated in frozen and paraffin-embedded tissue from 27 patients with malignant fibrous histiocytoma to elucidate the relationship between them, their implication in tumor progression mechanisms and their possible diagnostic-prognostic value in malignant fibrous histiocytoma. Single-strand conformation polymorphism analysis and direct sequencing of polymerase chain reaction-amplified DNA were used to establish two TP53 mutations (7.4%): a point mutation and a 63-bp duplication. Amplification of the MDM2 gene was observed in two tumors (7.4%) by means of Southern-blot analysis, one of them also carrying the TP53 point mutation. Immunohistochemical and Western-blot techniques were used to study nuclear accumulation of p53 and mdm2 proteins: 11 cases (40.7%) with p53 protein expression and thirteen cases (48.1%) with mdm2 protein expression were detected. We confirmed overexpression of mdm2 protein in eight of ten cases (80%) with p53 protein expression without TP53 gene mutation. Statistical analysis shows that simultaneous co-expression of p53 and mdm2 in malignant fibrous histiocytoma is significantly correlated with survival in absence of gene alteration in contrast to the lack of statistical correlation with survival of p53 protein expression alone.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Virchows Archiv 391 (1981), S. 107-115 
    ISSN: 1432-2307
    Schlagwort(e): Hemangioendothelioma ; Esophagus ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The histology and electron-microscopy of a malignant hemangioendothelioma of the esophagus wall appearing in a 42 year old male is presented. By light microscopy the tumor is composed of vessels and capillary-like structures of an anastomosing nature covered by atypical endothelial cells. These cells infiltrate the intersticial spaces growing into the posterior mediastinal area. Electron microscopy confirms the endothelial nature of the neoplastic cells, showing characteristics of the cell type, as is the presence of Weibel-Palade bodies, filaments and active pinocytosis. Hemangioendothelioma should be differentiated from other vascular tumors (angiosarcoma) as are hemangiopericytoma or hemangioblastoma, being composed exclusively of malignantly transformed endothelial cells.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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