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  • 1
    Electronic Resource
    Electronic Resource
    Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgaris L. (1980)
    Springer
    Planta 148 (1980), S. 397-406 
    Details
    ISSN: 1432-2048
    Keywords: Ethylene binding ; Hormone binding ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The preparation is described of a cell-free system from developing cotyledons of Phaseolus vulgaris cv. Canadian Wonder which is capable of binding ethylene. The binding is saturable and the apparent dissociation constant for ethylene is 6.4·10-10 M in solution. The binding site is associated with subcellular particles and treatment with Triton X-100 results in substantial solubilisation of the activity. The kinetics of association and dissociation of the ligand and the binding site are described. The system is heat labile and binding activity is diminished by treatment with some proteolytic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388129
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on ethylene binding by cell free preparations from cotyledons of Phaseolus vulgaris L. (1980)
    Springer
    Planta 148 (1980), S. 407-411 
    Details
    ISSN: 1432-2048
    Keywords: Ethylene binding ; Hormone binding ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various protein reactive agents such as dithioerythritol, dithiothreitol, mercaptoethanol and p-chloromercuribenzoate inhibit binding of ethylene to cell free preparations of Phaseolus vulgaris L. The effect of the thiols is partially reversed by treatment with diamide; occupation of the binding site by ligand diminishes the inhibition caused by p-chloromercuribenzoate but not that caused by thiols. Growth regulators other than ethylene do not affect binding. Physiologically active structural analogues of ethylene competitively inhibit binding of the growth regulator and their relative effectiveness in the cell free system closely resembles that in developmental processes controlled ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388130
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Ethylene binding in epicotyls of Pisum sativum L. cv. Alaska (1991)
    Springer
    Planta 183 (1991), S. 209-217 
    Details
    ISSN: 1432-2048
    Keywords: Ethylene binding ; Pisum (ethylene binding)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using in-vivo assays at least two classes of ethylene-binding site are shown to exist in pea epicotyls. These classes appear to have identical affinities for ethylene with a KD of 6 · 10−11-8 · 10−11 M; the fast associating class has high and appropriate affinities for physiologically active analogues of ethylene, and CO2 is without effect upon bfinding. Experiments involving suppression of endogenous ethylene production demonstrate that previous work may have underestimated both the amount of binding sites present and the affinity of such sites for ethylene. The apparent inhibition of binding by silver appears to be the consequence of a stimulation of endogenous ethylene production. The possible role of the sites as ethylene receptors is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197790
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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