ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

The evolution of the thrombospondin gene family (1993)

Lawler, Jack ; Duquette, Mark ; Urry, Lisa ; [et al.]

Springer

Journal of molecular evolution 36 (1993), S. 509-516

add to mindlist on the mindlist

Details

ISSN: 1432-1432

Keywords: Thrombospondin ; Evolution ; Adhesive glycoproteins

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Thrombospondin-1 is an adhesive glycoprotein that is involved in cellular attachment, spreading, migration, and proliferation. To date, four genes have been identified that encode for the members of the thrombospondin gene family. These four genes are homologous to each other in the EGF-like (type 2) repeats, the calcium-binding (type 3) motifs, and the COOH-terminal. The latter has been reported to be a cell-binding domain in thrombospondin-1. Phylogenetic trees have been constructed from the multisequence alignment of thrombospondin sequences from human, mouse, chicken, and frog. Two different algorithms generate comparable results in terms of the topology and the branch lengths. The analysis indicates that an early form of the thrombospondin gene duplicated about 925 million years ago. The gene duplication that produced the thrombospondin-1 and -2 branches of the family is predicted to have occurred 583 million years ago, whereas the gene duplication that produced the thrombospondin-3 and -4 branches of the family is predicted to have occurred 644 million years ago. These results indicate that the members of the thrombospondin gene family have existed throughout the evolution of the animal kingdom and thus probably participate in functions that are common to most of its members.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00556355

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Thrombospondin-4 is expressed by early osteogenic tissues in the chick embryo (1995)

Tucker, Richard P. ; Adams, Josephine C. ; Lawler, Jack

New York, NY [u.a.] : Wiley-Blackwell

Developmental Dynamics 203 (1995), S. 477-490

add to mindlist on the mindlist

Details

ISSN: 1058-8388

Keywords: Thrombospondin ; Development ; Extracellular matrix ; In situ hybridization ; Chondrogenesis ; Osteogenesis ; Cornea ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: The thrombospondins are a family of related glycoproteins found in the embryonic extracellular matrix. To date, five members of this family have been identified. Thrombospondin-1 and thrombospondin-2 have similar primary structure, but are expressed in different tissues at different times during development. Thrombospondins-3, -4, and cartilage oligomeric protein belong to a second thrombospondin subgroup in which the carboxyl-half of each molecule is most similar to thrombospondin-1 and -2. Here, we report the cloning and sequencing of a novel probe to avian thrombospondin-4. We have used this probe to determine the origins of thrombospondin-4 in the chick embryo by in situ hybridization. Thrombospondin-4 transcripts first appear in the mesenchyme surrounding bone anlage coinciding with the initial stages of osteogenesis. The expression in osteogenic tissues is transient: thrombospondin-4 mRNAs are not seen in the osteoblasts of bone collars in developing long bones. This pattern is distinct from avian thrombospondin-2, which is expressed in perichondrium and embryonic fibrous connective tissues. Our observations indicate that connective tissues are the principal site of thrombospondin-4 expression in the chick. The diverse origins of different thrombospondin gene family members imply distinctive roles for these proteins related to the growth and differentiation of cartilage, tendons, and bone. ©1995 Wiley-Liss, Inc.

Additional Material: 8 Ill.