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  • pumpkin (Cucurbita sp.)  (2)
  • FLUORESCENCE IN SITU HYBRIDIZATION  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Molecular characterization of a glyoxysomal citrate synthase that is synthesized as a precursor of higher molecular mass in pumpkin (1995)
    Springer
    Plant molecular biology 27 (1995), S. 377-390 
    Details
    ISSN: 1573-5028
    Keywords: amino-terminal presequence ; citrate synthase ; glyoxysome ; leaf peroxisome ; microbody transition ; pumpkin (Cucurbita sp.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone for glyoxysomal citrate synthase (gCS) was isolated from a λgt11 cDNA library prepared from etiolated pumpkin cotyledons. The cDNA of 1989 bp consisted of a 1548 bp open reading frame that encoded 516 amino acid residues. The deduced amino acid sequence of gCS did not have a typical peroxisomal targeting signal at its carboxyl terminal. A study of expression in vitro of the cDNA and an analysis of the amino-terminal sequence of the citrate synthase indicated that gCS is synthesized as a larger precursor that has a cleavable amino-terminal presequence of 43 amino acids. The predicted amino-terminal sequence of pumpkin gCS was highly homologous to those of other microbody enzymes, such as 3-ketoacyl-CoA thiolase of rat and malate dehydrogenase of watermelon that are also synthesized as precursors of higher molecular mass. Immunoblot analysis showed that the level of gCS protein increased markedly during germination and decreased rapidly during the light-induced transition of microbodies from glyoxysomes to leaf peroxisomes. By contrast, the level of mRNA for gCS reached a maximum earlier than that of the protein and declined even in darkness. The level of the mRNA was low during the microbody transition. These results indicate that the accumulation of the gCS protein does not correspond to that of the mRNA and that degradation of gCS is induced during the microbody transition, suggesting that post-transcriptional regulation plays an important role in the microbody transition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020191
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    cDNA cloning and expression of a gene for 3-ketoacyl-CoA thiolase in pumpkin cotyledons (1996)
    Springer
    Plant molecular biology 31 (1996), S. 843-852 
    Details
    ISSN: 1573-5028
    Keywords: glyoxysome ; leaf peroxisome ; microbody transition ; pumpkin (Cucurbita sp.) ; senescence ; 3-ketoacyl-CoA thiolase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone for 3-ketoacyl-CoA thiolase (EC 2.3.1.16) was isolated from a λgt11 cDNA library constructed from the poly(A)+ RNA of etiolated pumpkin cotyledons. The cDNA insert contained 1682 nucleotides and encoded 461 amino acid residues. A study of the expression in vitro of the cDNA and analysis of the amino-terminal sequence of the protein indicated that pumpkin thiolase is synthesized as a precursor which has a cleavable amino-terminal presequence of 33 amino acids. The amino-terminal presequence was highly homologous to typical amino-terminal signals that target proteins to microbodies. Immunoblot analysis showed that the amount of thiolase increased markedly during germination but decreased dramatically during the light-inducible transition of microbodies from glyoxysomes to leaf peroxisomes. By contrast, the amount of mRNA increased temporarily during the early stage of germination. In senescing cotyledons, the levels of the thiolase mRNA and protein increased again with the reverse transition of microbodies from leaf peroxisomes to glyoxysomes, but the pattern of accumulation of the protein was slightly different from that of malate synthase. These results indicate that expression of the thiolase is regulated in a similar manner to that of other glyoxysomal enzymes, such as malate synthase and citrate synthase, during seed germination and post-germination growth. By contrast, during senescence, expression of the thiolase is regulated in a different manner from that of other glyoxysomal enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019471
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Numerical Aberrations of Chromosomes 16, 17, and 18 in Hepatocellular Carcinoma (A FISH and FCM Analysis of 20 Cases) (1998)
    Springer
    Digestive diseases and sciences 43 (1998), S. 1-7 
    Details
    ISSN: 1573-2568
    Keywords: FLUORESCENCE IN SITU HYBRIDIZATION ; FLOW CYTOMETRY ; HEPATOCELLULAR CARCINOMA ; CHROMOSOME ABERRATION ; DNA PLOIDY
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Conventional cytogenetic studies havedemonstrated frequent abnormalities of specificchromosomes in hepatocellular carcinoma, although thereare few reports examining the relationship betweenchromosomal aberrations and clinicopathologic features. Inthis study, numerical aberrations of chromosomes 16, 17,and 18 were examined by fluorescence in situhybridization using pericentromeric DNA probes in 20 cases of surgically removed hepatocellularcarcinoma. DNA ploidy analysis was also performed byflow cytometry. Numerical abnormalities of chromosomes16, 17, and 18 were found in 7 of 19 cases, 15 of 20 cases, and 12 of 20 cases, respectively. Gainand/or loss of more than one chromosome was detected in16 of 19 cases. However, aneuploidy was seen in only 9of 20 tumors by flow cytometry. The incidence of aneusomy 17 and 18 increased with tumor sizeand stage progression. Fluorescence in situhybridization analysis demonstrated that numericalchromosomal aberrations accumulated with tumorprogression in hepatocellular carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018838731634
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    Paper (German National Licenses)
    Fulltext
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