ISSN:
1573-7330
Keywords:
IGFBP-1
;
IGFBP-3
;
FSH
;
human luteinizing granulosa cells
;
protease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Objectives To evaluate the regulation of the secretion of insulin-like growth factor (IGF) binding proteins (IGFBPs) by FSH in human luteinizing granulosa cells. Design and Results Luteinizing granulosa cells were incubated with and without FSH. Levels of IGFBP-1 and -3 in the medium were measured by EIA and RIA, respectively and binding activities of these IGFBP were evaluated by Western ligand blot. FSH inhibited the secretion of IGFBP-1 dose dependently. FSH did not inhibit the secretion of immunoreactive IGFBPS, but inhibited the binding activity of IGFBP-3. To assess the protease activity,125 I-IGFBP-3 was incubated with the cultured medium.125 I-IGFBP-3 degraded into small fragments when it was incubated with the cultured medium treated with FSH. Conclusions FSH enhances the action of IGF-I in human granulosa cells by inhibiting the secretion of IGFBP-1 and the binding activity of IGFBP-3 by stimulating the proteolysis of IGFBPS-3.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02212589
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