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  • 1
    ISSN: 1432-2242
    Keywords: Key words Potato breeding ; Family selection ; Cross prediction ; Breeders’ visual preference ; Within-family selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In 1992, 72 seedlings from each of 198 pair crosses were grown in a glasshouse, and the tubers produced by each plant were visually assessed on a 1–9 scale of increasing preference. Three groups of four progenies with high, medium and low mean scores were chosen to progress, without selection via tuber progenies and four-plant plots at a high-grade seed site, to replicated yield trials in the third clonal generation. The three groups maintained their high, medium and low scores for visual preference over the three clonal generations and also had high, medium and low scores in the second and third clonal generations for yield, size and appearance of tubers, all of which were components of visual preference. The three groups were predicted to have 13.6%, 1.8% and 0.2% of their clones exceeding the mean of 13 control cultivars for visual preference in the replicated trials, and 12.1%, 4.9% and 1.4% for yield, and 56.8%, 37.1% and 14.8% for appearance. The experiment confirmed that selection for visual preference within crosses in the seedling and first clonal generations is very ineffective, but that worthwhile progress can be made from selection in the second clonal generation, with correlated responses for faster emergence, earlier maturity, higher yield and greater regularity of shape (appearance). Combining selection of the high group of progenies with selection in the second clonal generation of the best 34 out of the 120 clones in this group, produced a response in visual preference in the third clonal generation of 1.00 compared with a maximum possible of 1.74. Ways of achieving further improvements in early-generation selection are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 68 (1984), S. 503-508 
    ISSN: 1432-2242
    Keywords: Kale breeding ; Population improvement ; Family selection ; Computer simulation ; Genetic drift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three recurrent selection schemes suitable for kale (Brassica oleracea L.), involving half-sib (HS), full-sib (FS) and selfed (S) families, were compared by computer simulation. All combinations of 6, 12 and 24 families selected, out of 120 and 240 assessed, were investigated for a range of genetical models. Selection was simulated for 20 generations from an initial allele frequency of 0.05 and for 16 generations from an initial frequency of 0.20. With an initial frequency of 0.05 there was a serious loss of desired alleles ranging from 0.31 out of 20 for the HS scheme with 24 out of 240 families selected to 9.19 for the S scheme with 6 out of 120 families selected. It was concluded that if as many as 20 cultivars were included in the initial population the selection scheme should be chosen to minimise the loss. With an initial frequency of 0.20 there were no losses with 12 and 24 families selected in the HS and FS schemes respectively, and the highest loss was 2.88 for the S scheme with 6 out of 120 families selected. It was concluded that if as few as five cultivars were included in the initial population a compromise between the initial response to selection and the loss of desired alleles should be sought. Selecting 6, 12 and 24 families for the HS, FS and S schemes respectively, resulted in average relative responses per generation of 2.28, 2.74 and 2.76, respectively for the first five generations, and losses of 0.22, 0.13 and 0.35, respectively after 16 generations. Practical considerations favour the FS scheme over the S scheme.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Key words Potato ; Solanum tuberosum ; Nematode resistance ; Globodera pallida ; Linkage in autotetraploids ; Quantitative trait locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Seventy eight clones from the cross between SCRI clone 12601ab1 and cv Stirling were used to explore the possibility of genetical linkage analysis in tetraploid potato (Solanum tuberosum subsp. tuberosum). Clone 12601ab1 had quantitative resistance to Globodera pallida Pa2/3 derived from S. tuberosum subsp. andigena. The strategy adopted involved identifying single- (simplex) and double- (duplex) dose AFLP markers in the parents from segregation ratios that could be unambiguously identified in their offspring, detecting linkage between a marker and a putative quantitative trait locus (QTL) for resistance, and placing the QTL on the linkage map of markers. The numbers of scorable segregating markers were 162 simplex ones present only in 12601ab1, 87 present in Stirling, and 32 present in both; and 72 duplex markers present only in 12601ab1 and 45 present in Stirling. The total map length was 990.9 cM in 12601ab1 and 484.6 cM in Stirling. A QTL with a resistance allele present in double dose (QQqq) in 12601ab1 was inferred from the associations between resistance scores (square root of female counts) and two duplex markers linked in coupling, which, in turn, were linked in coupling to four simplex markers also associated with resistance, but to a lesser degree. The largest marker class difference was the one for the duplex marker P61M34=15. It accounted for 27.8% of the phenotypic variance in resistance scores, or approximately 30% of the genotypic variance. Subsequently, this duplex marker was found to be linked in coupling with a duplex SSR allele Stm3016=a, whose locus was shown to be on chromosome IV in a diploid reference mapping population. The other QTLs for resistance segregating in the progeny were not identified for one or more of the following reasons: the markers did not cover the whole of the genome, there were unfavourable repulsion linkages between the QTLs and markers, or the gene effects were not large enough to be detected in an experiment of the size conducted. It is concluded that prospects appear good for detecting QTLs and using marker-assisted selection in a tetraploid potato breeding programme, provided that, in future, the population size is increased to over 250 and more SSR markers are used to complement the AFLPs; the same is likely to be true for other autotetraploid crops.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1617-4623
    Keywords: Key words AFLP ; Linkage map ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have constructed a partial linkage map in tetraploid potato which integrates simplex, duplex and double-simplex AFLP markers. The map consists of 231 maternal and 106 paternal markers with total map lengths of 990.9 cM and 484.6 cM. The longer of the two cumulative map lengths represents approximately 25% coverage of the genome. In tetraploids, much of the polymorphism between parental clones is masked by `dosage' which significantly reduces the number of individual markers that can be scored in a population. Consequently, the major advantage of using AFLPs – their high multiplex ratio – is reduced to the point where the use of alternative multi-allelic marker types would be significantly more efficient. The segregation data and map information have been used in a QTL analysis of late blight resistance, and a multi-allelic locus at the proximal end of chromosome VIII has been identified which contributes significantly to the expression of resistance. No late blight resistance genes or QTLs have previously been mapped to this location.
    Type of Medium: Electronic Resource
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