Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Doubly sensitivity-enhanced 3D TOCSY-HSQC (1996)
    Springer
    Journal of biomolecular NMR 8 (1996), S. 319-330 
    Details
    ISSN: 1573-5001
    Keywords: B0 gradients ; INEPT ; COS-INEPT ; Multidimensional NMR ; Sensitivity enhancement ; TOCSY-HSQC ; HSQC ; Flavodoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Recently, strategies for double sensitivity enhancement in heteronuclear three-dimensional NMR experiments were introduced (Krishnamurthy, V.V. (1995) J. Magn. Reson., B106, 170–177; Sattler et al. (1995) J. Biomol. NMR, 6, 11–22; Sattler et al. (1995) J. Magn. Reson., B108, 235–242). Since a sensitivity enhancement of a factor 21/2 can be achieved for each indirect dimension, nD spectra can theoretically be enhanced up to a factor of 2((n-1)/2). We propose and analyze a doubly enhanced three-dimensional TOCSY-HSQC sequence. The application of the doubly enhanced three-dimensional {15N, 1H} TOCSY-HSQC sequence is shown for uniformly 13C-/15N- and 15N-labeled samples of the relatively large Azotobacter vinelandii flavodoxin II (179 amino acids). The main factors that contribute to the final signal-to-noise enhancement have been systematically investigated. The sensitivity enhancement obtained for the doubly enhanced TOCSY-HSQC pulse sequence as compared to the standard (unenhanced) version is close to the theoretically expected factor of two.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410330
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A high-resolution HCANH experiment with enhanced sensitivity via multiple quantum line narrowing (1999)
    Springer
    Journal of biomolecular NMR 14 (1999), S. 169-174 
    Details
    ISSN: 1573-5001
    Keywords: calmodulin ; HCANH ; multiple quantum line narrowing ; NMR assignment ; resolution ; SEF2-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We report a 3D constant-time HCANH experiment (CTSL-HCANH) that uses the slower relaxation of multiple-quantum coherence to increase sensitivity and provides high Cα resolution. In this experiment the Hα of the (Hα, Cα) multiple quanta are selectively spin locked, so that Hα chemical shift evolution and 1 H-1H J-dephasing become ineffective during the relatively long delay needed for Cα to N coherence transfer. As compared to an HCANH experiment that uses Cα single-quantum coherence, an average enhancement of 20% was observed on calmodulin in complex with the binding domain of the transcription factor SEF2-1. Compared to CBCANH the signal intensity is approximately twice as good. The favorable relaxation properties of multiple quanta, together with the outstanding Cα resolution, make the experiment a very good complement to CBCANH and CBCA(CO)NH for sequential assignment of larger proteins for which deuteration is not yet necessary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008308307363
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...