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  • 1
    ISSN: 0009-8981
    Keywords: Interstitial collagenase ; Monoclonal antibody ; Sandwich enzyme immunoassay ; Tissue inhibitors of metalloproteinases
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0009-8981
    Keywords: Monoclonal antibody ; Sandwich enzyme immunoassay ; Tissue inhibitor of metalloproteinases-2
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 27 (1990), S. 152-158 
    ISSN: 1040-452X
    Keywords: Stallion spermatozoa ; Acrosome reaction ; Monoclonal antibody ; Zona-free hamster eggs ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The acrosome of the stallion spermatozoon was visualized by indirect immunofluorescence with monoclonal antibody (18.6) which recognized an integral acrosomal membrane component. Localization was confirmed by electron microscopy using peroxidase labelled antibody. In fresh semen samples (n = 19), 73.9 ± 9.1% of the spermatozoa from five fertile stallions displayed a uniform bright fluorescence over their acrosome region. In two semen samples from an infertile stallion only 28% and 35% of spermatozoa showed the same pattern of fluorescence. Spermatozoa from fertile stallions incubated for up to 12 hours in TALP medium maintained motility and exhibited a significant progressive loss of acrosomes as detected by immunofluorescence. Alternatively, a similar loss of acrosomes could be induced with calcium ionophore A23187 over a 90 minute incubation. Ultrastructural observations and incubation with zona-free hamster eggs indicated that only with ionophore treatment was immunofluorescent acrosome loss correlated with a physiological acrosome reaction, while prolonged sperm incubation led to degenerative membrane changes. It was concluded that, if carefully validated, immunofluorescent localization of the acrosome of stallion sperm with monoclonal antibody could be used to monitor the acrosome reaction. Furthermore, definitive acrosome visualization would be valuable in assessing semen quality.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 9 (1989), S. 1431-1451 
    ISSN: 0271-2091
    Keywords: Probabilistic diffusion-convection equation ; Gaussian field approach ; Random walk method ; Fluctuation Peclet number ; Porous media ; Two-phase system ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The channels formed between individual particles in porous media have variable dimensions and orientations. The porosity, permeability and its anisotropy exhibit random spatial distributions. The probabilistic approach can effectively describe the transport of contaminants through porous media and is analysed in this paper. Numerical results are obtained by considering (I) random dispersion coefficients without and with spatial structure, (II) random time distribution of concentration at the inlet boundary, (III) random velocity distribution in the flow field without and (IV) with variable dispersion coefficient, (V) non-linearity of the governing equation and (VI) anisotropy of the dispersion coefficient. Two methods are used for probabilistic predictions: (1) Gaussian field approach in conjunction with Monte Carlo method and (2) random walk method. The input random parameters are assumed to have normal and log-normal distributions according to available experimental data. The probability distribution functions of the contaminant concentration at different locations within the flow domain are calculated and compared with the input distributions as a function of the mean and fluctuation Peclet numbers. The one-dimensional case is analysed in detail and the illustrative numerical predictions are compared with analytical and experimental results. The extension to a two-dimensional domain is discussed in the last part of this paper.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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