Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Review of fluorescence anisotropy decay analysis by frequency-domain fluorescence spectroscopy (1993)
    Springer
    Journal of fluorescence 3 (1993), S. 103-116 
    Details
    ISSN: 1573-4994
    Keywords: Fluorescence anisotropy decays ; frequency-domain fluorescence spectroscopy ; review
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This didactic paper summarizes the mathematical expressions needed for analysis of fluorescence anisotropy decays from polarized frequency-domain fluorescence data. The observed values are the phase angle difference between the polarized components of the emission and the modulated anisotropy, which is the ratio of the polarized and amplitude-modulated components of the emission. This procedure requires a separate measurement of the intensity decay of the total emission. The expressions are suitable for any number of exponential components in both the intensity decay and the anisotropy decay. The formalism is generalized for global analysis of anisotropy decays measured at different excitation wavelengths and for different intensity decay times as the result of quenching. Additionally, we describe the expressions required for associated anisotropy decays, that is, anisotropy decays where each correlation time is associated with a decay time present in the anisotropy decay. And finally, we present expressions appropriate for distributions of correlation times. This article should serve as a reference for researchers using frequency-domain fluorometry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00865324
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Distance-dependent fluorescence quenching ofN-acetyl-l-tryptophanamide by acrylamide (1993)
    Springer
    Journal of fluorescence 3 (1993), S. 199-207 
    Details
    ISSN: 1573-4994
    Keywords: N-acetyl-l-tryptophanamide ; tryptophan fluorescence intensity decay ; frequency-domain fluorometry ; fluorescence spectroscopy ; distance-dependent quenching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We examined the time-dependent intensity decays ofN-acetyl-l-tryptophanamide (NATA) when collisionally quenched by acrylamide in propylene glycol over a range of temperatures. The intensity decays of NATA became increasingly heterogeneous in the presence of acrylamide. The NATA intensity decays were not consistent with the Collins-Kimball radiation boundary condition (RBC) model for quenching. The steady-state Stern-Volmer plots show significant upward curvature, and quenching of NATA by acrylamide was observed even in vitrified propylene glycol, where translational diffusion cannot occur during the lifetime of the excited state. These frequencydomain and steady-state data indicate a through-space quenching interaction between NATA and acrylamide, and the results are consistent with a rate constant for quenching that depends exponentially on the fluorophore-quencher separation distance. The exponential distance-dependent rate of quenching also explains the upward curvature of the Stern-Volmer plot, and the steady-state data aid in determining the interaction distance between NATA and acrylamide. These results suggest that the distance-dependent quenching rates need to be considered in the interpretation of acrylamide quenching of proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00862743
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...