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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 9 (1990), S. 226-230 
    ISSN: 1432-0789
    Keywords: Rhizosphere pseudomonads ; Fluorescent pseudomonads ; Ohgotrophs ; Selective media ; Phospholipid fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Diluted and selective media formulations were evaluated for the recovery of oligotrophic pseudomonads from the rhizosphere of the cucumber plant (Cucumis sativus L.). Full-strength King's B medium (KB) and 250-fold diluted medium (KB/250) were used as the basal formulations. The selective media formulations were: (1) Novobiocin, penicillin, and cycloheximide; (2) crystal violet and nitrofurantoin; and (3) Irgasan. On average, more rhizobacteria were recovered on the KB/250 than on the KB medium. For the recovery of pseudomonads, media made selective with either the novobiocin-penicillin-cycloheximide or the crystal violet and nitrofurantoin formulations were superior to the Irgasan formulation. Attempts to identify isolates using phospholipid fatty acid profiling met with limited success. The test isolates yielded mostly low similarity indices or “no matches” with the Microbial Identification System (aerobic library version) used for this study. All oligotrophic isolates were facultative and no evidence was obtained for the presence of obligate oligotrophs in these plating experiments.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0789
    Keywords: Biological suppression ; Pythium damping-off ; Rhizosphere pseudomonads ; Pseudomonas spp. ; Cucumis sativus L. ; Oligotrophs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pseudomonads recovered from the cucumber rhizosphere were evaluated for their ability to suppress Pythium ultimum damping-off. Full-strength and 250-fold diluted selective media formulations with King's B medium (KB and KB/250, respectively) as the basal medium were used as the recovery media. Eight per cent of the isolates recovered (11/138) could induce suppression to Pythium damping-off and 45% of these biocontrol agents produced fluorescent pigments. No differences in the ability to induce suppression among the isolates recovered on full-strength and diluted media were detected. The growth rates of the selected isolates indicated that growth in KB broth was faster or equal to that in KB/10. Therefore, no obligate oligotrophs were recovered. Additionally, growth was observed in 15 000-fold diluted KB for 10 of 11 test isolates, which indicated that these isolates were facultative oligotrophs. In general, the faster growing isolates were more likely to induce suppression when used as seed treatments. Finally, the in vitro antifungal properties of test isolates against P. ultimum indicated that 4 of the 11 isolates inhibited P. ultimum.
    Type of Medium: Electronic Resource
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